Silencing PPA1 inhibits human epithelial ovarian cancer metastasis by suppressing the Wnt/β-catenin signaling pathway

Oncotarget
Haiying NiuPengpeng Qu

Abstract

Inorganic pyrophosphatase (PPA1) activity is a key determinant of cellular inorganic pyrophosphate levels, and its expression is correlated with growth of several solid tumors. To investigate this relationship, we first examined PPA1 expression in human epithelial ovarian cancer (EOC) samples, and found that PPA1 was overexpressed in tumors from EOC patients. Higher PPA1 levels correlated with advanced grades, stages, and poor survival in EOC patients. Examination of PPA1 function in EOC revealed that silencing PPA1 inhibited EOC migration, epithelial-mesenchymal transition (EMT), and metastasis in vitro and in vivo. In addition, PPA1 may promote the dephosphorylation and translocation of β-catenin. These results demonstrate that silencing PPA1 inhibits EOC metastasis by suppressing the Wnt/β-catenin signaling pathway. Strategies for downregulating PPA1 may have therapeutic potential for the prevention and treatment of EOC.

References

Nov 5, 1999·Biochimica Et Biophysica Acta·T A Fairchild, G Patejunas
Mar 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Takeshi TomonagaFumio Nomura
May 3, 2005·Nature Reviews. Cancer·Honami Naora, Denise J Montell
Aug 11, 2005·Molecular & Cellular Proteomics : MCP·Brian L HoodDavid B Krizman
Jan 27, 2009·Cancer Metastasis Reviews·Mahmut Yilmaz, Gerhard Christofori
Sep 8, 2009·Bone·Monika D PolewskiRobert Terkeltaub
Apr 5, 2011·Cell·Jonathan P Sleeman, Jean Paul Thiery
Mar 24, 2012·Journal of Cellular Biochemistry·Lina WangRong Xiang
Jul 17, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sang-Ho JeongSoon-Chan Hong
Jul 19, 2012·Journal of Cellular Biochemistry·Li-Na WangYi-Xuan Yang
Jan 22, 2013·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
May 17, 2014·Bioconjugate Chemistry·Karuna GiriPriyabrata Mukherjee

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Citations

Sep 26, 2019·Oncogenesis·Dehong LuoShuang Yang
May 16, 2019·Journal of Cellular Physiology·Sebastián BocchicchioGriselda Irusta
Jul 18, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Dehong LuoWenzhi Shen

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Methods Mentioned

BETA
nuclear translocation
xenografts
xenograft
hysterectomy
confocal microscopy
transfection
Assay

Software Mentioned

Image J

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