Silencing Stem Cell Factor Gene in Fibroblasts to Regulate Paracrine Factor Productions and Enhance c-Kit Expression in Melanocytes on Melanogenesis

International Journal of Molecular Sciences
Pin-Hui LiHui-Min David Wang

Abstract

Melanogenesis is a complex physiological mechanism involving various paracrine factors. Skin cells such as keratinocytes, fibroblasts, and melanocytes communicate with one another through secreted regulators, thereby regulating the melanocytes' bio-functions. The stem cell factor (SCF) is a paracrine factor produced by fibroblasts, and its receptor, c-kit, is expressed on melanocytes. Binding of SCF to c-kit activates autophosphorylation and tyrosine kinase to switch on its signal transmission. SCF inhibition does not suppress fibroblast proliferation in MTT assay, and SCF silencing induced mRNA expressions of paracrine factor genes, HGF, NRG-1, and CRH in qPCR results. Following UVB stimulation, gene expressions of HGF, NRG, and CRH were higher than homeostasis; in particular, HGF exhibited the highest correlation with SCF variations. We detected fibroblasts regulated SCF in an autocrine-dependent manner, and the conditioned medium obtained from fibroblast culture was applied to treat melanocytes. Melanogenesis-related genes, tyrosinase and pmel17, were upregulated under conditioned mediums with SCF silencing and exposed to UVB treatments. Melanin quantities in the melanocytes had clearly increased in the pigment content assay...Continue Reading

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Citations

Aug 31, 2019·Environmental and Molecular Mutagenesis·Kelly L HarrisBarbara L Parsons
Mar 4, 2020·International Journal of Molecular Sciences·Yu Ri WooHyun Jeong Park
Apr 4, 2020·Journal of the European Academy of Dermatology and Venereology : JEADV·Ridam KapoorDavinder Parsad
Jun 9, 2020·Experimental and Therapeutic Medicine·Wenhui QianHua Su
Sep 11, 2020·Oxidative Medicine and Cellular Longevity·Hsing-Yu HaungHui-Min David Wang
Nov 16, 2019·International Journal of Molecular Sciences·Minjeong KimKyung-Min Lim
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·SeoYeon ShinKyungMok Park
Oct 17, 2020·Oxidative Medicine and Cellular Longevity·Jian LiHui-Min David Wang

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Methods Mentioned

BETA
transfection
gene knockdown
Assay
PCR

Software Mentioned

Mreg

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