Silencing the Metallothionein-2A gene induces entosis in adherent MCF-7 breast cancer cells

The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology
Yiyang LaiBoon-Huat Bay

Abstract

The presence of a live cell cohabiting within another cell has fascinated scientists for many decades. Far from being a spurious event, many have attempted to uncover the molecular mechanism underlying this phenomenon. In this study, we observed anchorage-dependent MCF-7 cells internalizing neighboring epithelial cells (entosis) after siRNA-mediated silencing of the Metallothionein-2A (MT-2A) gene. MTs belong to a family of low-molecular weight proteins, which bind metal ions endogenously and its over-expression has been reported in a variety of cancers that include breast, prostate, and colon. We provide microscopic evidence at light and ultrastructural levels of the occurrence of entosis after altering MT expression in a subpopulation of MCF-7 breast cancer cells by silencing the MT-2A gene. Our results demonstrate that adheren junctions may play important roles in the formation of cell-in-cell cytostructure after MT-2A gene downregulation and the entotic process does not appear to involve genes associated with autophagy. Interiorized cells often underwent lysosomal degradation within the cytoplasmic body of the engulfing cell. It would appear that a subset of breast cancer cells could die via entosis after MT-2A gene silencing.

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Citations

Oct 12, 2012·Drug Metabolism Reviews·Sona KrizkovaRene Kizek
Jan 25, 2012·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Yue-Qin Yang, Ji-Cheng Li
Jul 12, 2017·ELife·Joanne DurganOliver Florey
Sep 5, 2020·Cancers·Izabela Mlynarczuk-BialyLukasz P Bialy
Aug 25, 2018·Journal of Hematology & Oncology·Manfei Si, Jinghe Lang
Aug 18, 2021·IScience·Ruoyao ChenMichael Overholtzer

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