Silent Witness: Dual-Species Transcriptomics Reveals Epithelial Immunological Quiescence to Helminth Larval Encounter and Fostered Larval Development

Frontiers in Immunology
Friederike EbnerSusanne Hartmann

Abstract

Gastrointestinal nematodes are among the most prevalent parasites infecting humans and livestock worldwide. Infective larvae of the soil-transmitted nematode Ascaris spp. enter the host and start tissue migration by crossing the intestinal epithelial barrier. The initial interaction of the intestinal epithelium with the parasite, however, has received little attention. In a time-resolved interaction model of porcine intestinal epithelial cells (IPEC-J2) and infective Ascaris suum larvae, we addressed the early transcriptional changes occurring simultaneously in both organisms using dual-species RNA-Seq. Functional analysis of the host response revealed an overall induction of metabolic activity, without induction of immune responsive genes or immune signaling pathways and showing suppression of chemotactic genes like CXCL8/IL-8 or CHI3L1. Ascaris larvae, when getting in contact with the epithelium, showed induction of genes that orchestrate motor activity and larval development, such as myosin, troponin, myoglobin, and protein disulfide isomerase 2 (PDI-2). In addition, excretory-secretory products that likely facilitate parasite invasion were increased, among them, aspartic protease 6 or hyaluronidase. Integration of host and ...Continue Reading

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Citations

Jan 31, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ruizhe WangWen Yuan
Oct 18, 2020·Trends in Parasitology·Javier González-MiguelMar Siles-Lucas
Oct 4, 2020·Trends in Parasitology·Ankur MidhaSusanne Hartmann

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Datasets Mentioned

BETA
PRJNA450204

Methods Mentioned

BETA
RNA-Seq
PCR

Software Mentioned

Limma
psych R package
Blast2GO
SignalP
TopHat split - mapper
FastQC
biomaRt
GEN2
featureCounts
BlastKOALA

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Ascariasis is a helminthic infection of global distribution with more than 1.4 billion persons infected throughout the world. Here is the latest research.

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