PMID: 25773855Mar 17, 2015Paper

Silibilin-induces apoptosis in breast cancer cells by modulating p53, p21, Bak and Bcl-XL pathways

Asian Pacific Journal of Cancer Prevention : APJCP
Mohammad Bagher PirouzpanahNasser Samadi

Abstract

Nowadays herbal-derived medicines are attracting attention as new sources of drugs with few side effects. Silibinin is a flavonoid compound with chemotheraputic effects on different cancers such as examples in the prostate, lung, colon and breast. In the present study, the cytotoxic effects of silibinin on MCF7 breast cancer cells were investigated. Apoptosis was determined by flow cytometry and the impact of silibinin on the expression of pivotal genes including Bak, P53, P21, BRCA1, BCL-X1 and ATM was analyzed. Treatment for 24h had a significant dose-dependent inhibitory effect on cell growth (p<0.05) with dose- and time- dependent induction of apoptosis (p<0.05). In addition, there were significant increases in BRCA1, ATM, Bak and Bcl-XL gene expression at the mRNA level with different concentrations of silibinin for 24 or 48 h (p<0.05). Taken together, the results suggest that silibinin inhibits the proliferation and induces apoptosis of MCF-7 cells by down-regulating Bak, P53, P21, BRCA1, BCL-Xl and thus may be considered as an effective adjuvant drug to produce a better chemopreventive response for the cancer therapy.

References

Mar 29, 2005·The Journal of Biological Chemistry·Sivanandhan DhanalakshmiRajesh Agarwal
Apr 26, 2008·Molecular Nutrition & Food Research·Sonia Ramos
Jul 23, 2011·Apoptosis : an International Journal on Programmed Cell Death·Henriette KauntzFrancis Raul
Aug 19, 2011·Journal of breast cancer·Eun-Mi NohSung Hoo Jung
Jan 31, 2012·Free Radical Research·Simiao FanTakashi Ikejima
May 11, 2013·Nutrition and Cancer·Reza GhasemiArdeshir Ghavamzadeh
Jul 28, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Marzieh NasiriAbbas Alibakhshi
Jul 22, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Mehdi SabzichiNasser Samadi
Nov 7, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Simin SharifiNasser Samadi
Dec 19, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Mohammed I AlhazmiAli A Alshatwi

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Citations

Feb 10, 2016·BioMed Research International·Chen-Yi LiaoWu-Hsien Kuo
Jul 1, 2016·Expert Review of Clinical Pharmacology·Xing-Xing ZhuQiang He
Dec 29, 2016·Pharmaceutical Biology·Ommoleila MolaviRaymond Lai
Dec 15, 2020·Human Gene Therapy·Motahareh Sheikh-HosseiniBabak Arjmand

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