Silica colloidal crystals as three-dimensional scaffolds for supported lipid films

Langmuir : the ACS Journal of Surfaces and Colloids
Eric E Ross, Mary J Wirth

Abstract

This report describes the assembly of laterally diffusive lipid layers within the pores of colloidal crystals for potential application in membrane-based sensing. The amount of lipid encapsulated within colloidal crystals depends upon the method used to introduce the lipid to the crystalline substrate. Relative to a planar supported lipid bilayer, lipid loading in a 6.6 microm thick crystal was 15-73 times greater, as observed by fluorescence microscopy. Protein adsorption studies indicate that the crystal pores are open and that the silica surface of the crystal is passivated with respect to adsorption of a model protein when coated with POPC. Furthermore, the mesoporous environment of the colloidal crystal is found to protect lipid films from drying and rehydration processes that destroy planar supported lipid bilayers. The potential of colloidal crystal encapsulated lipid films for chemical sensing is demonstrated by a model protein binding assay.

References

Nov 1, 1976·Biophysical Journal·D E KoppelW W Webb
Jan 30, 1991·Biochimica Et Biophysica Acta·R C MacDonaldL R Hu
Jun 12, 1986·Biochimica Et Biophysica Acta·H M McConnellA A Brian
Mar 20, 2002·Accounts of Chemical Research·Jay T Groves, Steven G Boxer
May 27, 2004·Journal of the American Chemical Society·Matthew A HoldenPaul S Cremer
Nov 2, 2004·Analytical Chemistry·Travis R BesangerJohn D Brennan
Mar 3, 2005·Journal of the American Chemical Society·Victoria YamazakiJay T Groves
Mar 15, 2005·Analytical Chemistry·Jeremy P Walker, Sanford A Asher
May 19, 2005·Journal of the American Chemical Society·Michael R NewtonIlya Zharov
Jul 27, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Fernando AlbertorioPaul S Cremer
Nov 18, 2005·Journal of Colloid and Interface Science·Karin GlasmästarBengt Kasemo
Jan 5, 2006·Journal of the American Chemical Society·Adrian M BrozellAtul N Parikh
Jun 14, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Andrew K Bohaty, Ilya Zharov
Jun 22, 2006·Journal of the American Chemical Society·Julie Cichelli, Ilya Zharov
Apr 11, 2007·Analytical Chemistry·Suping ZhengMary J Wirth
Jun 28, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Thai Van LeMary J Wirth

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Citations

Jun 4, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Eric E RossSteven R Bugni
Aug 3, 2010·Acta Biomaterialia·Barbara A NellisSubhash H Risbud
Sep 22, 2009·Biochimica Et Biophysica Acta·Emel I GoksuMarjorie L Longo
May 18, 2016·Journal of Chromatography. a·Eric E RossSarah Owens
Jan 23, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Angela R Soemo, Mary J Wirth

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