Silica nanoparticles inhibit the cation channel TRPV4 in airway epithelial cells

Particle and Fibre Toxicology
Alicia SanchezKarel Talavera

Abstract

Silica nanoparticles (SiNPs) have numerous beneficial properties and are extensively used in cosmetics and food industries as anti-caking, densifying and hydrophobic agents. However, the increasing exposure levels experienced by the general population and the ability of SiNPs to penetrate cells and tissues have raised concerns about possible toxic effects of this material. Although SiNPs are known to affect the function of the airway epithelium, the molecular targets of these particles remain largely unknown. Given that SiNPs interact with the plasma membrane of epithelial cells we hypothesized that they may affect the function of Transient Receptor Potential Vanilloid 4 (TRPV4), a cation-permeable channel that regulates epithelial barrier function. The main aims of this study were to evaluate the effects of SiNPs on the activation of TRPV4 and to determine whether these alter the positive modulatory action of this channel on the ciliary beat frequency in airway epithelial cells. Using fluorometric measurements of intracellular Ca2+ concentration ([Ca2+]i) we found that SiNPs inhibit activation of TRPV4 by the synthetic agonist GSK1016790A in cultured human airway epithelial cells 16HBE and in primary cultured mouse tracheobron...Continue Reading

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Citations

Nov 18, 2018·The Journal of Pharmacology and Experimental Therapeutics·Raziye MohammadpourHamidreza Ghandehari
Jun 3, 2020·International Journal of Molecular Sciences·Tamara RosenbaumFrancisco Torres-Quiroz
Aug 20, 2019·Particle and Fibre Toxicology·Cristina PavanFrancesco Turci
Jul 11, 2019·British Journal of Pharmacology·Suhan YinLongquan Shao
Mar 24, 2020·Experimental & Molecular Medicine·George D LeikaufAn-Soo Jang
Jul 19, 2018·Particle and Fibre Toxicology·Sebastiano Di BucchianicoHanna L Karlsson
Oct 6, 2020·American Journal of Rhinology & Allergy·Kijeong LeeTae Hoon Kim
Apr 4, 2021·International Journal of Molecular Sciences·Alina Milici, Karel Talavera
Sep 28, 2020·International Journal of Biological Macromolecules·Janmejaya BagMonalisa Mishra
May 1, 2021·Cells·Suhasini RajanAlexander Dietrich

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Methods Mentioned

BETA
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Software Mentioned

WinASCD
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Clementine
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Clampex
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