Silica spheres coated with C18-modified gold nanoparticles for capillary LC and pressurized CEC separations

Electrophoresis
Qishu QuChao Yan

Abstract

Nonporous monodispersed silica spheres of 1.3 microm were coated with gold nanoparticles (AuNPs) and subsequently coated with n-octadecanethiol. By transmission electron microscopy analysis, the average diameter of the AuNPs on the silica spheres was determined to be 12 nm. The chromatographic and electrochromatographic properties of self-assembled n-octadecanethiol AuNP-coated silica microspheres (C18-AuNPs-SiO2) were investigated using a group of nonpolar PAHs. The stationary phase appears to display a characteristic reversed-phase behavior. Higher separation efficiency and shorter separation times were obtained using pressurized CEC (pCEC) compared with capillary LC (CLC). A maximum column efficiency of about 2.5x10(5) plates per meter and less than 18 min separation time for benzene were obtained in pCEC while only 66 507 plates per meter and an analysis time of nearly 100 min were observed in CLC mode. A chemical stability test of the C18-AuNPs-SiO2 stationary phase under extremely high and low pH conditions demonstrated that it is stable at pH 12 and 1 for at least 60 h. The results confirm that C18-AuNPs-SiO2 possesses a high rigidity to withstand high packing pressures and can be used as a good stationary phase for CLC ...Continue Reading

References

Aug 29, 2000·Journal of Chromatography. a·M M DittmannG P Rozing
Nov 28, 2001·Analytical Chemistry·B NeimanO Lev
Jan 31, 2002·Analytical Chemistry·M PumeraR Polsky
Mar 5, 2003·Journal of Chromatography. a·Kai ZhangChao Yan
Feb 19, 2004·Journal of Chromatography. a·J NawrockiP W Carr
Feb 19, 2004·Journal of Chromatography. a·J NawrockiP W Carr
Jan 5, 2005·Journal of Chromatography. a·H A Claessens, M A van Straten
Nov 30, 2005·Electrophoresis·Christian Nilsson, Staffan Nilsson
Jan 31, 2006·Journal of Chromatography. a·José M Cintrón, Luis A Colón
Jun 27, 2006·Journal of Chromatography. a·André de VilliersPat Sandra
Dec 1, 2006·Analytical Chemistry·Cheng-Ju YuWei-Lung Tseng
Jun 10, 2008·Journal of Chromatography. a·Qi-Shu QuChao Yan

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Citations

Nov 26, 2010·Analytical Chemistry·Bingchuan WeiMary J Wirth
Apr 19, 2011·Analytical Chemistry·Ana Ballesteros-Gómez, Soledad Rubio
Jun 15, 2013·The Analyst·Ekaterina P NesterenkoBrett Paull
Sep 19, 2015·Journal of Chromatography. a·Carlos E D NazarioFernando M Lanças
Aug 2, 2017·Electrophoresis·Sandya R BeeramDavid S Hage
Jul 7, 2017·Electrophoresis·Miguel Ángel González-CurbeloJavier Hernández-Borges

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