Silicon nanowire biosensor for highly sensitive and multiplexed detection of oral squamous cell carcinoma biomarkers in saliva

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Yulin ZhangGuo-Jun Zhang

Abstract

Silicon nanowire (SiNW) field-effect transistor (FET) biosensors have already been used as powerful sensors for the direct detection of disease-related biomarkers. However, the multiplexed detection of biomarkers in real samples is still challenging. Interleukin 8 (IL-8) and tumor necrosis factor α (TNF-α) are two typical biomarkers of oral squamous cell carcinoma (OSCC). In this study, we developed a multiplexed detection methodology for IL-8 and TNF-α detection in saliva using SiNW FET biosensors. We fabricated the SiNW FET sensors using a top-down lithography fabrication technique. Subsequently, we achieved the multiplexed detection of two biomarkers in saliva by specific recognition of the two biomarkers with their corresponding antibodies, which were modified on the SiNW. The established method was found to have a limit of detection as low as 10 fg/mL in 1 × PBS as well as 100 fg/mL in artificial saliva. Because of its advantages, including label-free and multiplexed detection, non-invasive analysis, highly sensitive and specific determination, the proposed method is expected to be widely used for the early diagnosis of OSCC.

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Citations

Nov 5, 2019·Mikrochimica Acta·Leila SyedmoradiKobra Omidfar
Jul 13, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Damla KiziltanEmir Baki Denkbas
Apr 21, 2020·Journal of Analytical Methods in Chemistry·Xinmei LiuYaru Wang
Nov 20, 2016·Journal of Cellular and Molecular Medicine·Karolina Elżbieta Kaczor-UrbanowiczDavid T W Wong
Feb 23, 2018·The Analyst·Puja Sandbhor Gaikwad, Rinti Banerjee
Jul 30, 2020·Analytical and Bioanalytical Chemistry·Dipti RaniVivek Pachauri
Oct 31, 2020·Research : a Science Partner Journal·Ruitao LiuTianhong Cui
Jan 31, 2021·Biosensors & Bioelectronics·Jasmeen KaurVivek Borse
Feb 28, 2021·Molecular Biotechnology·Jaya SinghalDivya Mehrotra
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuri D IvanovAlexander I Archakov
May 18, 2018·Analytical Chemistry·Thomas S BronderMichael J Schöning

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