Silicon nitride waveguide platform for fluorescence microscopy of living cells

Optics Express
Jean-Claude TinguelyBalpreet Singh Ahluwalia

Abstract

Waveguide chip-based microscopy reduces the complexity of total internal reflection fluorescence (TIRF) microscopy, and adds features like large field of view illumination, decoupling of illumination and collection path and easy multimodal imaging. However, for the technique to become widespread there is a need of low-loss and affordable waveguides made of high-refractive index material. Here, we develop and report a low-loss silicon nitride (Si3N4) waveguide platform for multi-color TIRF microscopy. Single mode conditions at visible wavelengths (488-660 nm) were achieved using shallow rib geometry. To generate uniform excitation over appropriate dimensions waveguide bends were used to filter-out higher modes followed by adiabatic tapering. Si3N4 material is finally shown to be biocompatible for growing and imaging living cells.

References

Oct 9, 2007·Biophysical Journal·Colin Echeverría AitkenJoseph D Puglisi
Apr 1, 2009·Optics Express·Björn AgnarssonKristjan Leosson
Nov 24, 2011·Optics Express·Björn AgnarssonKristjan Leosson
Dec 25, 2012·Optics Express·Johann RiemensbergerTobias J Kippenberg
Aug 20, 2015·Lab on a Chip·Firehun Tsige Dullo, Olav Gaute Hellesø
Dec 25, 2015·Optics Express·Firehun Tsige DulloOlav Gaute Hellesø

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Citations

Feb 19, 2020·Journal of Biophotonics·Ida S OpstadClemens F Kaminski
Mar 17, 2019·Optics Express·Øystein I HelleBalpreet S Ahluwalia
Sep 13, 2019·Optics Express·Florian StröhlClemens F Kaminski
Mar 22, 2019·Nature Communications·Anna ArchettiSuliana Manley
Mar 4, 2020·Optics Express·Daria KohlerChristian Koos
Dec 9, 2020·Communications Biology·Jean-Claude TinguelyBalpreet Singh Ahluwalia

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