Silk fibroin based biomimetic artificial extracellular matrix for hepatic tissue engineering applications

Biomedical Materials
Naresh Kasoju, Utpal Bora

Abstract

Hepatic tissue engineering, which aims to construct artificial liver tissues, requires a suitable extracellular matrix (ECM) for growth and proliferation of metabolically active hepatocytes. The current paper describes the development of a biomimetic artificial ECM, for hepatic tissue engineering applications, by mimicking the architectural features and biochemical composition of native ECM. Electrospinning was chosen as the fabrication technique of choice, while regenerated silk fibroin (RSF) and galactosylated chitosan (GalCS) were chosen as materials of choice. Poly(ethylene oxide) was used as a processing aid. Methodical optimization studies were performed to obtain smooth and continuous nanofibers with homogenous size distribution. Extensive characterization studies were performed to determine its morphological, physical, chemical/structural, thermal and cytotoxicity properties. Subsequently, detailed in vitro hepatocyte compatibility studies were performed using HepG2 cell line. Remarkably, the studies revealed that the growth, viability, metabolic activity and proliferation of hepatocytes were relatively superior on RSF-GalCS scaffold than on pure RSF and pure GalCS. In summary, the electrospun nanofibrous RSF-GalCS scaf...Continue Reading

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Citations

Sep 6, 2013·Tissue Engineering. Part B, Reviews·Ganesh C Ingavle, J Kent Leach
Jul 19, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Naresh Kasoju, Utpal Bora
Jan 6, 2016·Biomaterials Science·Naresh KasojuFritz Vollrath
Nov 28, 2012·Advanced Healthcare Materials·Naresh Kasoju, Utpal Bora
Apr 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pasquale SaccoIvan Donati
Apr 10, 2019·Polymers·Gabriela Carrasco-TorresAnaí A Valencia-Lazcano
Jul 22, 2019·Journal of Functional Biomaterials·Jimna Mohamed AmeerNaresh Kasoju
Nov 9, 2017·International Journal of Biological Macromolecules·Matteo CiocciSonia Melino
May 13, 2019·ACS Biomaterials Science & Engineering·Shreya MehrotraBiman B Mandal
Sep 5, 2020·Chemical Reviews·Adam M JorgensenAnthony Atala

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