Silk film culture system for in vitro analysis and biomaterial design.

Journal of Visualized Experiments : JoVE
Brian D LawrenceMark I Rosenblatt

Abstract

Silk films are promising protein-based biomaterials that can be fabricated with high fidelity and economically within a research laboratory environment (1,2). These materials are desirable because they possess highly controllable dimensional and material characteristics, are biocompatible and promote cell adhesion, can be modified through topographic patterning or by chemically altering the surface, and can be used as a depot for biologically active molecules for drug delivery related applications (3-8). In addition, silk films are relatively straightforward to custom design, can be designed to dissolve within minutes or degrade over years in vitro or in vivo, and are produce with the added benefit of being transparent in nature and therefore highly suitable for imaging applications (9-13). The culture system methodology presented here represents a scalable approach for rapid assessments of cell-silk film surface interactions. Of particular interest is the use of surface patterned silk films to study differences in cell proliferation and responses of cells for alignment (12,14). The seeded cultures were cultured on both micro-patterned and flat silk film substrates, and then assessed through time-lapse phase-contrast imaging, s...Continue Reading

References

Nov 7, 2002·Biomaterials·Mingzhong LiNorihiko Minoura
Nov 9, 2002·Biomaterials·Gregory H AltmanDavid L Kaplan
Jun 23, 2004·Biomaterials·Lorenz MeinelDavid L Kaplan
Nov 18, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Xilan MaHesun Zhu
Feb 7, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·S HofmannL Meinel
Mar 29, 2008·Biomacromolecules·Brian D LawrenceFiorenzo G Omenetto
Dec 9, 2008·Biomaterials·Brian D LawrenceDavid L Kaplan
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Diana E Jaalouk, Jan Lammerding
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Ning WangDonald E Ingber
Dec 25, 2009·Biomedical Microdevices·Anuj A PatelSanjay Kumar
Jan 2, 2010·Biomaterials·Keiji NumataDavid L Kaplan
Jan 30, 2010·Macromolecular Bioscience·Brian D LawrenceDavid L Kaplan
Oct 1, 2011·Nature Protocols·Danielle N RockwoodDavid L Kaplan

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Citations

Nov 28, 2012·PloS One·Brian D LawrenceMark I Rosenblatt
Nov 10, 2012·Advanced Drug Delivery Reviews·Banani KunduXungai Wang
Mar 14, 2014·Tissue Engineering. Part a·Corinna PetschBjörn O Bachmann
Jan 25, 2020·PloS One·Paul BelloVictor Parada
Aug 2, 2016·Current Protocols in Protein Science·Guoping RenMehmet Berkmen
Jan 10, 2020·ACS Applied Materials & Interfaces·G TulliiM R Antognazza

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Methods Mentioned

BETA
scanning electron imaging

Software Mentioned

CyQuant

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