Silver nanoparticle-induced phosphorylation of histone H3 at serine 10 is due to dynamic changes in actin filaments and the activation of Aurora kinases

Toxicology Letters
Xiaoxu ZhaoYuko Ibuki

Abstract

The phosphorylation of histone H3 at serine 10 (p-H3S10) has been closely correlated with mitotic chromosome condensation. We previously reported that silver nanoparticles (AgNPs) significantly induced p-H3S10 independent of mitosis. In the present study, we examined the mechanisms underlying the induction of p-H3S10 by AgNPs. A treatment with AgNPs markedly induced p-H3S10 in a dose-dependent manner in three types of cell lines, and this was dependent on the cellular incorporation of AgNPs. The immunofluorescent staining of AgNP-induced p-H3S10 was thin and solid throughout the nucleus, and differed from that normally associated with mitosis. AgNPs induced the formation of globular actin in a dose-dependent manner. Latrunculin B (LatB) and phalloidin, inhibitors of actin polymerization and depolymerization, respectively, inhibited p-H3S10, suggesting that dynamic changes in actin filaments are related to AgNP-induced p-H3S10. Furthermore, p-H3S10 was mediated by Aurora kinase (AURK) pathways, which were suppressed by LatB and siRNA for cofilin 1, an actin-depolymerizing protein. AgNO3 (Ag ions) exerted similar effects to those of AgNPs. These results suggest that Ag ions released from AgNPs incorporated into inner cells change...Continue Reading

Citations

Dec 7, 2019·Biomaterials Science·Jie YuJing Ruan
Oct 17, 2019·Frontiers in Bioengineering and Biotechnology·Mallikarjuna Rao GeddaPrasoon Madhukar
Jul 14, 2018·International Journal of Molecular Sciences·Shana J CameronWilliam G Willmore
Sep 28, 2020·Environmental Science and Pollution Research International·Michal ŠtefančíkMonika Pávková Goldbergová
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Jan 8, 2021·Journal of Nanobiotechnology·Marta Pogribna, George Hammons
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