Silver nanoparticles induced alterations in multiple cellular targets, which are critical for drug susceptibilities and pathogenicity in fungal pathogen (Candida albicans )

International Journal of Nanomedicine
Venkatraman Srinivasan RadhakrishnanTulika Prasad

Abstract

A significant increase in the incidence of fungal infections and drug resistance has been observed in the past decades due to limited availability of broad-spectrum antifungal drugs. Nanomedicines have shown significant antimicrobial potential against various drug-resistant microbes. Silver nanoparticles (AgNps) are known for their antimicrobial properties and lower host toxicity; however, for clinical applications, evaluation of their impact at cellular and molecular levels is essential. The present study aims to understand the cellular and molecular mechanisms of AgNp-induced toxicity in a common fungal pathogen, Candida albicans. AgNps were synthesized by chemical reduction method and characterized using UV-visible spectroscopy, X-ray powder diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive X-ray spectroscopy, energy dispersive X-ray fluorescence, and zeta potential. The anti-Candida activity of AgNps was assessed by broth microdilution and spot assays. Effects of AgNps on cellular and molecular targets were assessed by monitoring the intracellular reactive oxygen species (ROS) production in the absence and presence of natural antioxidant, changes in surface morphology, cellular ul...Continue Reading

Citations

Sep 30, 2020·World Journal of Microbiology & Biotechnology·Ifeanyi Elibe Mba, Emeka Innocent Nweze
Oct 6, 2020·Frontiers in Cellular and Infection Microbiology·Brenda KischkelCarlos P Taborda
May 23, 2020·Advanced Science·Samuel CheesemanAaron Elbourne
Apr 17, 2020·Frontiers in Bioengineering and Biotechnology·Thomas E PatersonJoanna Shepherd
Apr 26, 2019·The Journal of Antibiotics·Shivkrupa D HalbandgeSankunny Mohan Karuppayil
Feb 8, 2020·Medical Mycology·Júlia de Souza Silveira ValenteDaniela Isabel Brayer Pereira
Jan 1, 2019·Medical Mycology·Júlia de Souza Silveira ValenteDaniela Isabel Brayer Pereira
Apr 4, 2021·International Journal of Molecular Sciences·Clara Couto FernandezLjubica Tasic
May 25, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Dongmei Jia, Wenping Sun
May 27, 2021·Medical Mycology·Jhon Jhamilton Artunduaga BonillaLeonardo Nimrichter

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Methods Mentioned

BETA
X-ray
Fluorescence
Scanning
transmission electron microscopy
surface plasmon resonance
confocal microscopy

Software Mentioned

GraphPad
GraphPad Prism
PANalytical
IGOR6
’ Pert collector
LUDOX
Image Tool
Epsilon

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