Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations

Scientific Reports
Jennifer A FraserEva Malone

Abstract

Neutrophil surveillance is central to nanoparticle clearance. Silver nanoparticles (AgNP) have numerous uses, however conflicting evidence exists as to their impact on neutrophils and whether they trigger damaging inflammation. Neutrophil's importance in innate defence and regulating immune networks mean it's essential we understand AgNP's impact on neutrophil function. Human neutrophil viability following AgNP or Ag Bulk treatment was analysed by flow cytometry and AnV/PI staining. Whilst AgNP exposure did not increase the total number of apoptotic neutrophils, the number of late apoptotic neutrophils was increased, suggesting AgNP increase transit through apoptosis. Mature (CD16bright/CD62Lbright), immature (CD16dim/CD62Lbright) and apoptotic (CD16dim/CD62Ldim) neutrophil populations were evident within isolated neutrophil preparations. AgNP exposure significantly reduced CD62L staining of CD16bright/CD62Lbright neutrophils, and increased CD16 staining of CD16dim/CD62Lbright populations, suggesting AgNPs trigger neutrophil activation and maturation, respectively. AgNP exposure dramatically increased IL-8, yet not classical pro-inflammatory cytokine release, suggesting AgNP triggers neutrophil activation, without pro-inflammat...Continue Reading

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Citations

Jul 31, 2019·Cell Death & Disease·Sandeep KeshavanBengt Fadeel

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Methods Mentioned

BETA
flow cytometry
profiler
ELISA
light microscopy
dynamic light scattering
scraping
enzyme-linked immunosorbent assay

Software Mentioned

GraphPad Prism
ImageJ
REACH
Graphpad

Related Concepts

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