Silver Nanoparticles: Two-Faced Neuronal Differentiation-Inducing Material in Neuroblastoma (SH-SY5Y) Cells

International Journal of Molecular Sciences
Ahmed Abdal DayemSsang-Goo Cho

Abstract

We have previously demonstrated the potential of biologically synthesized silver nanoparticles (AgNP) in the induction of neuronal differentiation of human neuroblastoma, SH-SY5Y cells; we aimed herein to unveil its molecular mechanism in comparison to the well-known neuronal differentiation-inducing agent, all-trans-retinoic acid (RA). AgNP-treated SH-SY5Y cells showed significantly higher reactive oxygen species (ROS) generation, stronger mitochondrial membrane depolarization, lower dual-specificity phosphatase expression, higher extracellular-signal-regulated kinase (ERK) phosphorylation, lower AKT phosphorylation, and lower expression of the genes encoding the antioxidant enzymes than RA-treated cells. Notably, pretreatment with N-acetyl-l-cysteine significantly abolished AgNP-induced neuronal differentiation, but not in that induced by RA. ERK inhibition, but not AKT inhibition, suppresses neurite growth that is induced by AgNP. Taken together, our results uncover the pivotal contribution of ROS in the AgNP-induced neuronal differentiation mechanism, which is different from that of RA. However, the negative consequence of AgNP-induced neurite growth may be high ROS generation and the downregulation of the expression of the...Continue Reading

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Citations

Sep 12, 2019·International Journal of Molecular Sciences·Sangiliyandi GurunathanJin-Hoi Kim
Mar 15, 2019·International Journal of Molecular Sciences·Caroline E Nunes-XavierRafael Pulido
Apr 3, 2020·International Journal of Molecular Sciences·Zannatul Ferdous, Abderrahim Nemmar
Jan 18, 2020·Scientific Reports·Tahereh EsmaeilpourVahid Salari
Aug 11, 2020·Reviews in the Neurosciences·Hamidreza ArzaghiHamidreza Pazoki-Toroudi

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Methods Mentioned

BETA
transmission
dynamic light scattering
PCR
protein assay
electrophoresis
X-ray

Software Mentioned

GraphPad InStat
NeuriteTrace
GraphPad
ImageJ
Excel

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