Silver stains demonstrating neuroendocrine cells

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
L Grimelius

Abstract

During the preimmunohistochemical era, silver stains were an important part of the staining arsenal for identifying certain tissue structures and cell types in tissue sections. Some of them were useful for demonstrating endocrine cells, especially in the gastrointestinal tract. Until the late 1950s, silver stains, particularly those identifying endocrine cells, were accompanied by a number of technical difficulties resulting from uncontrolled staining factors. In the 1960s, new silver stains were developed for endocrine cell types and these stains gave reproducible results. One of the "older" silver stains and two of the "newer" ones are emphasized in this presentation, namely the Masson, the Grimelius and the Sevier-Munger techniques. The Masson stain demonstrates the enterochromaffin (EC, serotonin) cells, the Grimelius stain is a broad endocrine cell marker, and the Sevier-Munger technique demonstrates EC and EC-like cells and the C-cells of the thyroid. Especially in the preimmunohistochemical era, these staining methods often were used for histopathological diagnosis, particularly the Grimelius technique. The silver stains were developed empirically, and with few exceptions the chemical background is not known. Staining pr...Continue Reading

References

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Jan 1, 1955·The Journal of Pathology and Bacteriology·R BARTER, A G E PEARSE
Jan 1, 1965·Journal of Neuropathology and Experimental Neurology·A C SEVIER, B L MUNGER

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Citations

Jul 4, 2015·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·D StefanovićD Lalošević
Sep 5, 2009·Autonomic Neuroscience : Basic & Clinical·Paul P Bertrand, Rebecca L Bertrand
Mar 15, 2016·Developmental and Comparative Immunology·Tryntsje CuperusHenk P Haagsman
Aug 10, 2007·Pathology International·Michiko Tanaka-Shintani, Makoto Watanabe
Jun 6, 2009·Veterinary Clinical Pathology·Katie BoesJonathan Miller
Jun 10, 2015·Archives of Environmental Contamination and Toxicology·Dércia SantosSandra Mariza Monteiro
Jul 19, 2017·Chemical Biology & Drug Design·Hailong ShiYifei Qin
Jun 10, 2010·Folia Histochemica Et Cytobiologica·Sandra Mariza MonteiroMário Sousa
Nov 1, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Gustavo A Ramírez, Antonio Espinosa de Los Monteros
Nov 11, 2008·Upsala Journal of Medical Sciences·Lars Grimelius

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