Silver(I), mercury(II), cadmium(II), and zinc(II) target exposed enzymic iron-sulfur clusters when they toxify Escherichia coli.

Applied and Environmental Microbiology
Fang Fang Xu, J A Imlay

Abstract

The toxicity of soft metals is of broad interest to microbiologists, both because such metals influence the community structures in natural environments and because several metals are used as antimicrobial agents. Their potency roughly parallels their thiophilicity, suggesting that their primary biological targets are likely to be enzymes that contain key sulfhydryl moieties. A recent study determined that copper poisons Escherichia coli in part by attacking the exposed [4Fe-4S] clusters of dehydratases. The present investigation sought to test whether other soft metals also target these enzymes. In vitro experiments revealed that low-micromolar concentrations of Ag(I) and Hg(II) directly inactivated purified fumarase A, a member of the dehydratase family. The enzyme was also poisoned by higher levels of Cd(II) and Zn(II), but it was unaffected by even millimolar concentrations of Mn(II), Co(II), Ni(II), and Pb(II). Electron paramagnetic resonance analysis and measurements of released iron confirmed that damage was associated with destruction of the [4Fe-4S] cluster, and indeed, the reconstruction of the cluster fully restored activity. Growth studies were then performed to test whether dehydratase damage might underlie toxicit...Continue Reading

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S I Liochev, I Fridovich
Oct 1, 1990·Archives internationales de physiologie et de biochimie·B RajannaC S Chetty
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·K Keyer, J A Imlay
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·C RensingB P Rosen
Mar 27, 1998·Journal of Bacteriology·A S Gort, J A Imlay
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·C Alén, A L Sonenshein
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·C RensingB P Rosen
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Feb 8, 2002·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·K Hantke
Jun 28, 2003·FEMS Microbiology Reviews·Dietrich H Nies
May 17, 2005·Current Medicinal Chemistry·M ValkoM T D Cronin
Jun 30, 2005·Journal of the American Chemical Society·John S MagyarHilary Arnold Godwin
Nov 15, 2006·The Journal of Biological Chemistry·Soojin Jang, James A Imlay
Jul 24, 2007·The Journal of Biological Chemistry·Caroline RanquetMarc Fontecave
Oct 16, 2008·Biotechnology Advances·Mahendra RaiAniket Gade
Dec 6, 2008·Science·Ariel D Anbar
Dec 17, 2008·Nature Reviews. Microbiology·Kevin J Waldron, Nigel J Robinson
Mar 14, 2009·Current Opinion in Microbiology·Anne O Summers
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Lee Macomber, James A Imlay
Jul 10, 2009·The Journal of Biological Chemistry·Jeffry M LeitchValeria C Culotta
Apr 9, 2010·FEMS Microbiology Reviews·Robert Wysocki, Markus J Tamás
Sep 30, 2010·Metal Ions in Life Sciences·Michael AschnerSandra Ceccatelli
Jan 5, 2011·Applied and Environmental Microbiology·Gregor GrassMarc Solioz
Jan 22, 2011·Metallomics : Integrated Biometal Science·Susana M QuintalNicholas P Farrell
Mar 19, 2011·Toxicology·Klaudia Jomova, Marian Valko
Mar 23, 2011·Journal of Clinical Nursing·Michelle Beattie, Julie Taylor
Jul 30, 2011·Metallomics : Integrated Biometal Science·Lee Macomber, Robert P Hausinger
Aug 2, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Hengyi XuHua Wei
Sep 29, 2011·Metallomics : Integrated Biometal Science·F Barras, M Fontecave
Oct 11, 2011·Metallomics : Integrated Biometal Science·Christopher L DupontChristopher Rensing

❮ Previous
Next ❯

Citations

Mar 16, 2013·Chemical Reviews·Sonja EckhardtKatharina M Fromm
Jul 17, 2012·Nature Reviews. Microbiology·M Indriati Hood, Eric P Skaar
Jun 21, 2013·Science Translational Medicine·Jose Ruben Morones-RamirezJames J Collins
Apr 9, 2013·Applied and Environmental Microbiology·Lina J BirdDianne K Newman
Sep 26, 2013·Future Microbiology·Nadezhda GermanChristopher Rensing
Jan 29, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Zishuo ChengHuangen Ding
Mar 29, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Yong WeiZhengwei Fu
Sep 17, 2013·Applied and Environmental Microbiology·Sergios A NicolaouEleftherios T Papoutsakis
Jan 22, 2015·International Journal of Molecular Sciences·Humberto Palza
Jan 26, 2016·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Aniket GadeAnne J Anderson
Jun 20, 2014·Molecular Microbiology·Guoqiang TanHuangen Ding
Nov 25, 2014·Journal of Medical Microbiology·Jon L Hobman, Lisa C Crossman
May 11, 2013·Biochimica Et Biophysica Acta·Béatrice RocheFrédéric Barras
Jan 10, 2013·Biochimica Et Biophysica Acta·Béatrice RocheFrédéric Barras
Jun 7, 2013·Bioscience Reports·Sian L StaffordMatthew J Sweet
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Smitha PillaiKristin Schirmer
Nov 16, 2014·Applied and Environmental Microbiology·Alexandra JungMartin Thanbichler
Mar 6, 2016·Journal of Medical Microbiology·Joshua RavensdaleKeith Gregg
Sep 7, 2016·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Ruby YnalvezHector Gonzalez-Cantu
Jan 26, 2017·Environmental Science & Technology·S Carolina LondonoLynda B Williams
Mar 28, 2017·Nature Reviews. Microbiology·Pete ChandrangsuJohn D Helmann
Apr 7, 2017·Molecular Microbiology·Marion HerisseBenjamin Ezraty
Jun 10, 2017·Nature Communications·Seung-Hwan ChoiJung-Hye Roe
Oct 8, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Li CuiKaisong Zhang
Nov 10, 2017·International Journal of Molecular Sciences·Charlie J PyleLarry S Schlesinger
Dec 2, 2017·Nature Communications·Deenah OsmanNigel J Robinson
Feb 16, 2018·Oxidative Medicine and Cellular Longevity·Laurence VernisMeng-Er Huang

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