Silviavirus phage ɸMR003 displays a broad host range against methicillin-resistant Staphylococcus aureus of human origin

Applied Microbiology and Biotechnology
Chanthol PengYasunori Tanji

Abstract

The emergence of life-threatening methicillin-resistant Staphylococcus aureus (MRSA) has led to increased interest in the use of bacteriophages as an alternative therapy to antibiotics. The success of phage therapy is greatly dependent on the selected phage possessing a wide host range. This study describes phage ɸMR003 isolated from sewage influent at a municipal wastewater treatment plant in Tokyo, Japan. ɸMR003 could infect 97% of 104 healthcare- and community-associated MRSA strains tested, compared with 73% for phage ɸSA012, which has a broad host range against bovine mastitis S. aureus. Genome analysis revealed that ɸMR003 belongs to the genus Silviavirus which has not been studied extensively. ɸMR003 recognizes and binds to wall teichoic acid (WTA) of S. aureus during infection. In silico comparisons of the genomes of ɸMR003 and ɸSA012 revealed that ORF117 and ORF119 of ɸMR003 are homologous to the putative receptor-binding proteins ORF103 and ORF105 of ɸSA012, with amino acid similarities of 75% and 72%, respectively. ORF104, which is an N-acetylglucosaminidase found in the ɸMR003 tail, may facilitate phage's infection onto the WTA-null S. aureus RN4220. The differences in tail and baseplate proteins may be key contribu...Continue Reading

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Citations

May 4, 2021·Comprehensive Reviews in Food Science and Food Safety·Zhenhua HuangYong Zhao
May 18, 2021·Frontiers in Molecular Biosciences·Lauren WalshR Paul Ross
Jun 22, 2021·Frontiers in Microbiology·Erick Adrian Cruz-LópezVirgilio Bocanegra-García
Aug 28, 2021·Antibiotics·Aa Haeruman AzamLongzhu Cui

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Datasets Mentioned

BETA
AP019522

Methods Mentioned

BETA
transmission electron microscopy
PCR
electrophoresis
restriction modification

Software Mentioned

Velvet
HHpred
Basic Local Alignment Search Tool ( BLAST )
EMBOSS Stretcher
VICTOR Building
BLAST
POT
Remus

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