Silylated precision particles for controlled release of proteins

ACS Applied Materials & Interfaces
Khosrow KhodabandehlouJoseph M DeSimone

Abstract

With the recent advances in the development of novel protein based therapeutics, controlled delivery of these biologics is an important area of research. Herein, we report the synthesis of microparticles from bovine serum albumin (BSA) as a model protein using Particle Replication in Non-wetting Templates (PRINT) with specific size and shape. These particles were functionalized at room temperature using multifunctional chlorosilane that cross-link the particles to render them to slowly-dissolving in aqueous media. Mass spectrometric study of the reaction products of diisopropyldichlorosilane with individual components of the particles revealed that they are capable of reacting and forming cross-links. Energy dispersive spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS) were also used to confirm the functionalization of the particles. Cross sectional analysis using focused ion beam (FIB) and EDS proved that the functionalization occurs throughout the bulk of the particles and is not just limited to the surface. Circular dichroism data confirmed that the fraction of BSA molecules released from the particles retains its secondary structure thereby indicating that the system can be used for delivering protein based formu...Continue Reading

References

Apr 22, 1992·International Journal of Cancer. Journal International Du Cancer·P RivaG Franceschi
Mar 21, 1989·Biochemistry·K Srinivasachar, D M Neville
Oct 14, 1988·Biochemical and Biophysical Research Communications·R E Benesch, S Kwong
Jul 1, 1984·The Journal of Pharmacy and Pharmacology·J H RatcliffeS S Davis
Jan 12, 2000·Acta Chemica Scandinavica·G HouenV Barkholt
Apr 12, 2001·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·P Costa, J M Sousa Lobo
Apr 25, 2003·International Journal of Pharmaceutics·K LangerD Schubert
Jun 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mark X YangAlexey L Margolin
Dec 16, 2003·Annals of the Rheumatic Diseases·S N NikasA A Drosos
Apr 6, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sergey PechenovAlexey L Margolin
Nov 22, 2005·Expert Opinion on Drug Delivery·Larry R Brown
Oct 10, 2006·The AAPS Journal·Amy S Rosenberg
Nov 14, 2006·Advanced Drug Delivery Reviews·Davis Yohanes ArifinChi-Hwa Wang
Nov 23, 2006·Journal of Microencapsulation·Henry NetteyMartin J D'Souza
Jun 5, 2007·Advanced Drug Delivery Reviews·António J Almeida, Eliana Souto
Apr 2, 2008·Journal of the American Chemical Society·Jennifer Y Kelly, Joseph M DeSimone
Jun 28, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Felix Kratz
Aug 16, 2008·Journal of Pharmaceutical Sciences·John F CarpenterBarry Cherney
Sep 22, 2009·Trends in Biotechnology·Zuzana AntosovaTomas Macek
Oct 7, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Maria A MillerKeith P Johnston
Feb 10, 2010·Pharmaceutical Research·Mark Cornell ManningDerrick S Katayama
Mar 24, 2010·Journal of Pharmaceutical Sciences·Satish K SinghThomas Spitznagel
Apr 14, 2010·PloS One·Nishit Doshi, Samir Mitragotri
Nov 26, 2010·Journal of the American Chemical Society·Matthew C ParrottJoseph M Desimone
Feb 22, 2012·Journal of Microencapsulation·Carl W Oettinger, Martin J D'Souza
Feb 22, 2012·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Seiji OhtoriKazuhisa Takahashi
Mar 1, 2012·Chemical Reviews·Tina VermondenWim E Hennink
Mar 1, 2012·International Journal of Pharmaceutics·Alexandra RollettGeorg M Guebitz
May 2, 2012·Journal of the American Chemical Society·Matthew C ParrottJoseph M DeSimone
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri

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Citations

Feb 3, 2016·Advanced Healthcare Materials·Khosrow KhodabandehlouJoseph M DeSimone
Aug 25, 2018·Pharmaceutical Research·Erin M WilsonJoseph M DeSimone

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