Silymarin-Loaded Eudragit Nanoparticles: Formulation, Characterization, and Hepatoprotective and Toxicity Evaluation

AAPS PharmSciTech
Amira E El-NahasAmal H El-Kamel

Abstract

The objectives of this study were to formulate, characterize silymarin-loaded Eudragit nanoparticles (SNPs) and evaluate their hepatoprotective and cytotoxic effects after oral administration. SNPs were prepared by nanoprecipitation technique and were evaluated for particle size, entrapment efficiency, TEM, solid-state characterization, and in vitro drug release. The hepatoprotective activity was evaluated after oral administration of selected SNPs in carbon tetrachloride-intoxicated rats. Potential in vivo acute cytotoxicity study was also assessed. The selected SNPs contained 50 mg silymarin and 50 mg Eudragit polymers (1:1 w/w Eudragit RS 100 & Eudragit LS 100). Morphology of the selected SNPs (particle size of 84.70 nm and entrapment efficiency of 83.45% with 100% drug release after 12 h) revealed spherical and uniformly distributed nanoparticles. DSC and FT-IR studies suggested the presence of silymarin in an amorphous state and absence of chemical interaction. The hepatoprotective evaluation of the selected SNPs in CCl4-intoxicated rats revealed significant improvement in the activities of different biochemical parameters (P ≤ 0.01) compared to the marketed product. The histopathological studies suggested that the selecte...Continue Reading

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Citations

Jun 12, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alfonso Di Costanzo, Ruggero Angelico
Sep 1, 2020·International Journal of Pharmaceutics·Helissara Silveira DiefenthaelerIrene Clemes Külkamp-Guerreiro
May 3, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Marina França DiasArmando Silva Cunha

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Methods Mentioned

BETA
nanoprecipitation
dynamic light scattering
transmission electron microscopy
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differential scanning calorimetry
MDA

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DD
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