PMID: 9420262Jan 7, 1998Paper

Simian immunodeficiency virus (SIV) envelope-specific Fabs with high-level homologous neutralizing activity: recovery from a long-term-nonprogressor SIV-infected macaque

Journal of Virology
J GlamannVanessa M Hirsch

Abstract

An antibody phage display library was constructed from RNA extracted from lymph node cells of a simian immunodeficiency virus (SIV)-infected long-term-nonprogressor macaque. Seven gp120-reactive Fabs were obtained by selection of the library against SIV monomeric gp120. Although each of the Fabs was unique in sequence, there were two distinct groups based on epitope recognition, neutralizing activity in vitro, and molecular analysis. Group 1 Fabs did not neutralize SIV and bound to a linear epitope in the V3 loop of the SIV envelope. In contrast, two of the group 2 Fabs neutralized homologous, neutralization-sensitive SIVsm isolates with high efficiency but failed to neutralize heterologous SIVmac isolates. Based on competition enzyme-linked immunosorbent assays with mouse monoclonal antibodies of known specificity, these Fabs reacted with a conformational epitope that includes domains V3 and V4 of the SIV envelope. These neutralizing and nonneutralizing Fabs provide valuable standardized and renewable reagents for studying the role of antibody in preventing or modifying SIV infection in vivo.

References

Apr 1, 1992·European Journal of Immunology·N P WengD M Marcus
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K JavaherianD P Bolognesi
Jul 1, 1992·European Journal of Immunology·T H WinklerJ R Kalden
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D R BurtonR A Lerner
Dec 5, 1991·Journal of Molecular Biology·J D MarksG Winter
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C F BarbasS J Benkovic
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M Ivey-HoyleM Rosenberg
Jan 1, 1990·Annual Review of Immunology·R C Desrosiers
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T J PalkerB F Haynes
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K JavaherianS D Putney
Jul 1, 1995·The Journal of Experimental Medicine·Q J Sattentau, J P Moore
Jul 1, 1995·AIDS Research and Human Retroviruses·M GardnerE Robinson
Apr 15, 1995·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·A MuñozJ P Phair
May 1, 1995·Immunology Today·G P Cook, I M Tomlinson
Aug 1, 1994·AIDS·S P BuchbinderS D Holmberg
Dec 1, 1993·AIDS Research and Human Retroviruses·C J ScandellaB Chapman
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·D J BenosE N Benveniste
Jun 1, 1996·The Journal of Infectious Diseases·K K Van RompayM L Marthas

❮ Previous
Next ❯

Citations

Aug 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Margit H HansenHenrik J Ditzel
May 21, 2016·Proteomics. Clinical Applications·Philipp KuhnMichael Hust
Oct 27, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Darren J SchofieldRobert H Purcell
Oct 26, 1999·Viral Immunology·A N VzorovR W Compans
Sep 12, 2014·Clinical and Vaccine Immunology : CVI·Virginie S AdamMaria P Limberis
Sep 22, 2010·AIDS Research and Human Retroviruses·Takeo KuwataTatsuhiko Igarashi
Jul 6, 2004·Journal of Microbiological Methods·Valery A Petrenko, Iryna B Sorokulova
Jul 27, 2021·Frontiers in Cellular and Infection Microbiology·Kristian Daniel Ralph RothMichael Hust

❮ Previous
Next ❯

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.