Similar effects of osmolarity, glucose, and phosphate on cleavage past the 2-cell stage in mouse embryos from outbred and F1 hybrid females

Biology of Reproduction
Timin HadiJay M Baltz

Abstract

One-cell-stage embryos derived from most random-bred and inbred female mice exhibit an in vitro developmental block at the two-cell stage in classical embryo culture media. However, embryos derived from many F1 hybrids develop easily past the two-cell stage under the same conditions. This has given rise to the commonly accepted idea that there exist blocking and nonblocking types of female mice, with only the former being prone to a two-cell block. Recently, culture media have been improved to the point that even embryos prone to the two-cell block will develop past the block in vitro, making it possible to study its etiology. Here, we show that either increased osmolarity or increased glucose/phosphate levels induced the expected two-cell block in random-bred CF1 embryos and the two-cell block at increased osmolarities could be rescued by the organic osmolyte glycine. Surprisingly, one-cell embryos from B6D2F1 (BDF1) F1 hybrid females, considered to be nonblocking, also became blocked at the two-cell stage when osmolarity or glucose/phosphate levels were increased. They were also similarly rescued by glycine from the osmolarity-induced block. The most evident difference was that the purportedly nonblocking embryos became block...Continue Reading

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Citations

Oct 1, 2013·Journal of Assisted Reproduction and Genetics·Jay M Baltz
Dec 25, 2007·Reproduction, Fertility, and Development·Michelle LaneDeirdre L Zander-Fox
Oct 15, 2009·Human Reproduction Update·Jay M Baltz, Alina P Tartia
Jun 6, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Xiangpeng DaiQi Zhou
Jun 17, 2009·Seminars in Cell & Developmental Biology·R DumollardK Swann
Mar 7, 2008·Fertility and Sterility·Martha Hentemann, Kjell Bertheussen
Jan 29, 2008·Fertility and Sterility·Paulo Marcelo PerinPaulo Hilário Nascimento Saldiva
Sep 27, 2012·Molecular Reproduction and Development·Jay M Baltz, Chenxi Zhou
Jul 6, 2014·The Journal of Reproduction and Development·Kazuchika Miyoshi, Yamato Mizobe
Sep 3, 2016·Journal of Cellular Physiology·Samantha RichardJay M Baltz
Dec 23, 2017·Journal of Assisted Reproduction and Genetics·Jason R HerrickRebecca L Krisher
Oct 18, 2006·Journal of Cellular Physiology·Mohamed-Kheir Idris AnasJay M Baltz
Nov 28, 2013·Reproduction, Fertility, and Development·Jason R HerrickRebecca L Krisher
Mar 7, 2014·Biology of Reproduction·Hannah E CorbettJay M Baltz
Jun 15, 2017·Journal of Developmental Origins of Health and Disease·D K Gardner, R L Kelley
Jul 7, 2018·International Journal of Biomaterials·Daisaku IwamotoKazuhiro Saeki
Jun 20, 2020·Journal of Assisted Reproduction and Genetics·Michael Charles Summers

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