Abstract
Pancreatic hyperstimulation with simultaneous duct obstruction does not cause the typical features of acute pancreatitis, therefore the role of an additional challenge, such as either ethanol intoxication or short-term ischemia, was studied. Alcoholic pancreatitis was induced in 28 rats by acute ethanol intoxication (0.25 LD50) and an obstruction/hyperstimulation mechanism (clip of the biliopancreatic duct for 20 min and intravenous stimulation with 5 U of cholecystokinin and secretin each). Ischemic pancreatitis was performed by obstruction/hyperstimulation and subsequent pancreatic ischemia by clamping the supplying arteries for 40 min. The macro- and microscopic alterations were evaluated and graded by a scoring system. Additionally, the pancreas was removed in 50% of the animals and the pancreatic acini were prepared. From those acini the intracellular enzymes trypsinogen, kallikreinogen, amylase, lipase, glucuronidase, and acidic phosphatases were determined. While obstruction/hyperstimulation, 40 min of ischemia, or ethanol alone did not induce acute pancreatitis, a combination of obstruction/hyperstimulation with either ethanol or ischemia resulted in acute pancreatitis in 68 and 60% of treated rats, respectively. Simila...Continue Reading
Citations
Dec 13, 2006·Bulletin of Experimental Biology and Medicine·Yu I BorodinE V Yankaite
Aug 18, 2005·American Journal of Surgery·Alexander PerezEdward E Whang
Dec 10, 1999·Journal of the American College of Surgeons·S RätyI Nordback
Jul 27, 2002·Pancreas·Jens M MayerHans G Beger
Apr 30, 2003·Pancreas·Uwe A WittelMarco Siech
Aug 21, 2013·Free Radical Research·J A ArmstrongD N Criddle
Mar 12, 2008·HPB : the Official Journal of the International Hepato Pancreato Biliary Association·Kim Hue SuChristopher Christophi
Oct 29, 2010·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·J GrauvogelJ Werner
May 16, 2007·ANZ Journal of Surgery·John C PapakostasMiltiadis I Matsagas
Aug 4, 2011·The British Journal of Surgery·O Sadr AzodiA Wolk
Nov 26, 1998·Experimental and Molecular Pathology·A AndrzejewskaG Jurkowska
Apr 20, 2004·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Jan W DlugoszUrszula Wereszczynska-Siemiatkowska
Oct 25, 2003·Pancreas·Markus M LerchBurkhard Krüger
Jun 12, 2019·International Journal of Molecular Sciences·Pedro Silva-VazJosé Guilherme Tralhão
Sep 26, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Marco SiechMax G Bachem
Feb 14, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Zhao LuFred S Gorelick