PMID: 9421491Feb 28, 1998Paper

Similarities and differences among 105 members of the Int family of site-specific recombinases

Nucleic Acids Research
Simone E Nunes-DübyA Landy

Abstract

Alignments of 105 site-specific recombinases belonging to the Int family of proteins identified extended areas of similarity and three types of structural differences. In addition to the previously recognized conservation of the tetrad R-H-R-Y, located in boxes I and II, several newly identified sequence patches include charged amino acids that are highly conserved and a specific pattern of buried residues contributing to the overall protein fold. With some notable exceptions, unconserved regions correspond to loops in the crystal structures of the catalytic domains of lambda Int (Int c170) and HP1 Int (HPC) and of the recombinases XerD and Cre. Two structured regions also harbor some pronounced differences. The first comprises beta-sheets 4 and 5, alpha-helix D and the adjacent loop connecting it to alpha-helix E: two Ints of phages infecting thermophilic bacteria are missing this region altogether; the crystal structures of HPC, XerD and Cre reveal a lack of beta-sheets 4 and 5; Cre displays two additional beta-sheets following alpha-helix D; five recombinases carry large insertions. The second involves the catalytic tyrosine and is seen in a comparison of the four crystal structures. The yeast recombinases can theoretically ...Continue Reading

References

Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·Y Kikuchi, H A Nash
Dec 1, 1992·Trends in Genetics : TIG·W M StarkD J Sherratt
Sep 11, 1992·Nucleic Acids Research·D K WaltonA G Atherly
Oct 11, 1992·Nucleic Acids Research·M Conrad, M D Topal
Jun 5, 1992·Journal of Molecular Biology·M C Serre, M Jayaram
Aug 1, 1991·Epidemiology and Infection·A Galazka
Apr 25, 1991·Proceedings of the National Academy of Sciences of the United States of America·M H LeeG F Hatfull
Jan 1, 1991·Ophthalmology·D McLeod
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J W ChenM Jayaram
Jan 1, 1990·Methods in Enzymology·G J Barton
Dec 22, 1989·Cell·D G SchatzD Baltimore
Sep 1, 1989·Plasmid·S R Kim, T Komano
May 1, 1989·Molecular and Cellular Biology·A A Amin, P D Sadowski
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R HoessK Abremski
Sep 1, 1987·Molecular & General Genetics : MGG·J MadonR Hütter
Oct 25, 1988·Nucleic Acids Research·R SpritzR King
Mar 1, 1988·The British Journal of Dermatology·S WaltonW J Cunliffe
Feb 1, 1986·Journal of Bacteriology·P Sadowski
Jan 1, 1986·Molecular & General Genetics : MGG·C Disqué-KochemR Eichenlaub

❮ Previous
Next ❯

Citations

Dec 8, 2007·Folia Microbiologica·J FarkasovskáA Godány
Feb 20, 2008·Archives of Microbiology·B J HensonS R Barnum
Dec 7, 2011·Current Microbiology·Jarmila Farkašovská, Andrej Godány
Jun 5, 2012·Molecular Genetics and Genomics : MGG·Ahmed AskoraTakashi Yamada
Apr 21, 2005·Extremophiles : Life Under Extreme Conditions·Andrew M KropinskiKen F Jarrell
Aug 10, 2006·Extremophiles : Life Under Extreme Conditions·Patrizia ContursiQunxin She
Sep 22, 2005·Virus Genes·Yi LiXunzhang Wang
Dec 23, 2003·Journal of Molecular Biology·Amy C Groth, Michele P Calos
Dec 4, 2002·Journal of Molecular Biology·Michael TekleSimone E Nunes-Düby
Jan 1, 2003·Journal of Molecular Biology·Mikhail Kolot, Ezra Yagil
Feb 1, 2003·Journal of Molecular Biology·Linda Lee, Paul D Sadowski
Feb 13, 2003·Journal of Molecular Biology·John A Lewis, Graham F Hatfull
May 28, 2003·Journal of Molecular Biology·Srisunder SubramaniamMark P Foster
Feb 13, 2001·FEMS Microbiology Letters·B S NieldH W Stokes
May 29, 2003·FEMS Microbiology Letters·Loubna Jouan, George Szatmari
Oct 19, 2000·Research in Microbiology·S A Sciochetti, P J Piggot
Sep 18, 2002·Research in Microbiology·Qunxin SheLanming Chen
Nov 15, 2002·Methods : a Companion to Methods in Enzymology·Kaushik Ghosh, Gregory D Van Duyne
Oct 6, 2001·Current Opinion in Microbiology·D A Rowe-Magnus, D Mazel
Oct 6, 2001·Current Opinion in Microbiology·B Hallet
Mar 6, 1999·Current Opinion in Microbiology·J W Golden, H S Yoon
Oct 20, 2005·Journal of Bioscience and Bioengineering·M TsudaK Furukawa
Oct 4, 2006·Biochemistry·Kathy A GelatoEnoch P Baldwin
Jul 17, 1998·Nature Structural Biology·K M ConnollyR T Clubb
Jul 16, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Gilles MichelJiing-Kuan Yee
Jul 14, 1998·Nature Biotechnology·F BuchholzA F Stewart
Jul 18, 2006·Nature Reviews. Microbiology·Didier Mazel
Jul 6, 2010·Nature Reviews. Microbiology·Rachel A F Wozniak, Matthew K Waldor
Oct 29, 1998·Molecular Microbiology·P PfisterT Leisinger

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