Similarities in diesel exhaust particles induced alterations in expression of cytochrome P-450 and glutathione S-transferases in rat lymphocytes and lungs

Xenobiotica; the Fate of Foreign Compounds in Biological Systems
Ankita SrivastavaDevendra Parmar

Abstract

Freshly prepared peripheral blood lymphocytes (PBL) are known to express cytochrome P450s (CYPs) and glutathione S-transferases (GSTs) involved in the bioactivation and detoxification of organic components of diesel exhaust particles (DEPs). To validate that blood lymphocyte expression profiles could be used as a biomarker to predict exposure to vehicular emissions, similarities in the alterations in the mRNA expression of CYPs and GSTs were studied in PBL and lungs of rats exposed to DEPs. Adult male Wistar rats were treated transtracheally with different doses of DEPs (3.75- or 7.5- or 15- or 30-mg/kg b.wt.). The animals were anaesthetized after 24 h and blood was drawn and lungs were taken out and processed. DEP produced a similar pattern of increase in the mRNA expression of CYPs (CYP1A1, 1A2, 1B1, 2E1), associated arylhydrocarbon receptor (Ahr) and arylhydrocarbon nuclear translocator (Arnt) and GSTs (GSTPi, GSTM1 and GSTM2) at all the doses in lungs and PBL. The protein expression of CYP1A1/1A2 and 2E1 and catalytic activity of CYPs and GSTs also showed a similar pattern of increase in blood lymphocyte and in lungs isolated from DEP treated rats. Our data indicating similarities in the alterations in the expression of car...Continue Reading

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Citations

Aug 10, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Anwar Jamal KhanDevendra Parmar
Jul 17, 2014·Journal of Immunotoxicology·Abbas BahariHesam Dehghani
Mar 21, 2017·Neoplasia : an International Journal for Oncology Research·Sanjay YadavLevi J Beverly

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