Similarity of salt influences on the pH of buffers, polyelectrolytes, and proteins

The Journal of Physical Chemistry. B
Alina E VoinescuW Kunz

Abstract

Changes in pH induced by the addition of electrolytes to buffers, polyelectrolytes (a polycarboxy polymethylene and a polyethyleneimine), and proteins (casein, whey, and lysozyme) solutions are explored systematically. The two buffer systems are triethanolamine/triethanolammonium chloride and citric acid/sodium citrate. These are chosen because of the similarity of their acid-base equilibria with those of amino acids predominant in most proteins, that is, amino acids that include carboxylate or ammonium groups in their structures. The pH of triethanolamine and of citrate buffers respectively increases and decreases when salt is added. At low electrolyte concentrations (<0.15 mol/kg), the phenomenon is well accounted for by standard electrostatic theories. pH values at higher salt concentrations are not reliable when measured with a commercial glass electrode without cross-checking by a standard hydrogen electrode. The changes of the pH values of polyelectrolyte and protein solutions with added salts turn out to be remarkably similar to the salt induced pH changes in the buffer solutions. It is even possible to qualitatively predict these changes in protein solutions simply from the primary protein structure. At least in the sys...Continue Reading

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Citations

Jan 19, 2012·Chemical Reviews·Pierandrea Lo Nostro, Barry W Ninham
May 19, 2007·Journal of Biomolecular Structure & Dynamics·Marta AiroldiMauro Giustini
Feb 12, 2014·The Journal of Chemical Physics·Ruiting Zhang, Wei Zhuang
Jul 28, 2013·Journal of Colloid and Interface Science·Julien MarcusWerner Kunz
Jan 29, 2013·Colloids and Surfaces. B, Biointerfaces·Luyan XueYinbo Qu
Nov 7, 2015·The Journal of Physical Chemistry. B·Florian PlattenStefan U Egelhaaf
May 16, 2015·Chemosphere·Pierandrea Lo NostroPiero Baglioni
Nov 30, 2016·Journal of Pharmaceutical Sciences·Teddy J ZbacnikMark Cornell Manning
Aug 14, 2020·The Journal of Physical Chemistry. B·Barry W Ninham, Pierandrea Lo Nostro

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