Simple adhesive-tape-based sampling of tomato surfaces combined with rapid fluorescence in situ hybridization for Salmonella detection.

Applied and Environmental Microbiology
Bledar Bisha, Byron F Brehm-Stecher

Abstract

A simple adhesive-tape-based method for sampling of tomato surfaces was combined with fluorescence in situ hybridization for rapid culture-independent detection of Salmonella strains. Tapes could also be placed face-down on selective agar for on-tape enrichment of captured Salmonella cells. Overlay of cell-charged tapes with small volumes of liquid enrichment media enabled subsequent detection of tape-captured Salmonella via flow cytometry.

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Citations

Nov 5, 2010·Journal of Visualized Experiments : JoVE·Bledar Bisha, Byron F Brehm-Stecher
Feb 14, 2019·Future Microbiology·Tamara K KakoschkeFrank Ebel
Sep 3, 2009·Journal of Food Protection·Byron Brehm-StecherMary Lou Tortorello
Feb 12, 2020·Analytical and Bioanalytical Chemistry·Stephanie A HiceByron F Brehm-Stecher
Jan 30, 2018·Frontiers in Microbiology·Elena L Peredo, Sheri L Simmons

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