Simple adult rabbit model for Vibrio cholerae and enterotoxigenic Escherichia coli diarrhea.

Infection and Immunity
W M SpiraJ Froehlich

Abstract

We developed an adult rabbit model for enteric infection by Vibrio cholerae and enterotoxigenic Escherichia coli. The cecum of each animal was first ligated to prevent it from retaining fluid secreted by the small intestine. A temporary reversible obstruction (a slip knot tie) of the small bowel was introduced at the time of challenge and removed 2 h later. With this modification, we were able to elicit a massive and usually fatal cholera-like diarrhea in adult (3.5- to 6-lb [1.6- to 2.7-kb]) animals challenged with V. cholerae. Animals challenged with enterotoxigenic E. coli also developed diarrhea which was severe and watery but less explosive and less rapidly fatal than that produced by V. cholerae. The susceptibility of animals in this model to infection by V. cholerae was similar to the susceptibility of infant rabbits challenged intraintestinally. The death rate was almost 25% when 10(3) Vibrio cells were given and 90% or more when the dose was greater than or equal to 10(6) cells per animal. We designated this procedure the RITARD (for removable intestinal tie-adult rabbit diarrhea) model.

References

Nov 1, 1978·Infection and Immunity·V S BaselskiC D Parker
Apr 1, 1974·The Journal of Infectious Diseases·U BlachmanM J Pickett
Feb 1, 1969·The Journal of Infectious Diseases·R B Sack, C C Carpenter
Feb 1, 1969·The Journal of Infectious Diseases·R B Sack, C C Carpenter
Jan 1, 1970·Infection and Immunity·H Kusama, J P Craig

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Citations

Oct 1, 1989·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J E CollinsD S Shoop
Apr 10, 2010·Journal of Food Protection·N HaddadJ-M Cappelier
Jan 1, 2014·Applied and Environmental Microbiology·Donna L RunftJeffrey H Withey
Jun 11, 2017·Applied and Environmental Microbiology·Jiazheng WangBiao Kan
Jun 20, 2017·Applied and Environmental Microbiology·Kristie C MitchellJeffrey H Withey
Jun 21, 2019·The American Journal of Tropical Medicine and Hygiene·Mohammad Monir ShahYoshio Ichinose
Feb 11, 2020·Frontiers in Immunology·Saeideh Davoodi, Edan Foley
Jan 1, 1991·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·J A Davis, R E Banks
Apr 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Shah M FaruqueJohn J Mekalanos
Aug 10, 2012·Expert Review of Vaccines·Weiping Zhang, David A Sack
Jan 1, 1989·Immunological Investigations·M K EstesD Y Graham
Feb 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Shah M FaruqueJohn J Mekalanos
Apr 1, 1987·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·Y López-VidalA M Svennerholm
Dec 1, 1991·Digestive Diseases and Sciences·R N Greenberg
Sep 1, 1992·Acta Paediatrica. Supplement·M E Penny
Jun 10, 2010·FEMS Immunology and Medical Microbiology·Nivedita RoyHemanta Koley
Oct 26, 1999·Infection and Immunity·S M FaruqueJ J Mekalanos
Aug 12, 2020·Journal of Bacteriology·Dhrubajyoti NagJeffrey H Withey
Apr 1, 1991·Infection and Immunity·M J AlbertS Tzipori
Jul 1, 1997·Clinical Microbiology Reviews·T M Wassenaar
Mar 1, 1996·Microbiological Reviews·C L Sears, J B Kaper
Jan 1, 1995·Clinical Microbiology Reviews·J B KaperM M Levine
Dec 1, 1992·Infection and Immunity·M J AlbertJ Montanaro

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