Simple and efficient method for isolating cDNA fragments of lea3 genes with potential for wide application in the grasses (Poaceae)

Genetics and Molecular Research : GMR
L YuB Yan

Abstract

cDNA fragments of lea3 genes with a high GC content (from 68 to 77%) were found in several Poaceae, including Sorghum vulgare, Saccharum officinarum, Oryza officinalis, Oryza meyeriana, Ampelocalamus calcareus, Cynodon dactylon, and Zizania latifoli. They were successfully isolated by means of optimal experimental parameters, which included dimethyl sulfoxide as additive and degenerate primers "AGETKAS" and "AGKDKTG", and their sequences were analyzed. Compared to the method of isolating genes by screening of a cDNA library using abscisic acid- and other stress-responsive cDNA clones, which is time-consuming and costly, this method is relatively easy and inexpensive. Using this new method, many new homologue lea3 genes were rapidly determined.

References

Dec 25, 1990·Nucleic Acids Research·G SarkarS S Sommer
Mar 1, 1993·The Plant Journal : for Cell and Molecular Biology·L Dure
Jun 28, 2000·Nucleic Acids Research·M Kovárová, P Dráber
Dec 15, 2000·Plant Physiology·N Federspiel
Apr 2, 2005·Plant Molecular Biology·Ming-der ShihYue-Ie C Hsing
Nov 30, 2005·Nucleic Acids Research·Min LiHsiao-Sheng Liu

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