Simple and efficient oligonucleotide-directed mutagenesis using one primer and circular plasmid DNA template

Gene Analysis Techniques
K R Marotti, C S Tomich

Abstract

A rapid and simple procedure for site-directed mutagenesis is described. This method uses only a single oligonucleotide primer with the double-stranded circular plasmid DNA as the template for mutagenesis. The phage T4 gene 32 product is included during primer extension in vitro to increase efficiency. Single and multiple changes as well as deletions have been obtained at an efficiency of 1-2%.

References

Jan 1, 1987·Gene·K Foss, W H McClain
Jan 1, 1985·Annual Review of Genetics·M Smith
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D N BremsC S Tomich
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W Mandecki
Apr 1, 1980·Journal of Molecular Biology·M J Casadaban, S N Cohen

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