Simple and precise method for counting ciliary beats directly from the TV monitor screen

Journal of Pharmacological Methods
P C BragaL Allegra

Abstract

The modern optical microscopic technique together with the use of the small perfusion chamber allows the maintenance and observation of isolated ciliated epithelia for many hours in vitro. Counting ciliary beats is a good means for evaluating the behavior of these cells immediately under different environmental conditions and also for evaluating the pharmacologic or toxic action of drugs. Using a TV camera joined to a microscope equipped with the Nomarsky differential interference contrast set, it is possible to see on a TV monitor an image of the cilia at a very high magnification (over 1000 X), with a good resolution. This paper describes a method and an instrument, with a digital readout purposely designed to receive and count signals from a phototransistor placed directly on the protective screen of the TV monitor to correspond exactly with the point of passage of the coming and going movement of the selected cilia. In this way, it is possible to obtain (in real time and in a simple way through visual observation of the beat) the final count, which consists of the number of passages, in a prefixed time, in front of the phototransistors of the dark area which represents the ciliary body on the light background.

References

Mar 1, 1977·IEEE Transactions on Bio-medical Engineering·H Shimizu, G Matsumoto
Jul 1, 1974·Acta Oto-laryngologica·U MerckeN G Toremalm
Dec 20, 1974·Nature·M DuboisG David
Nov 1, 1972·The Journal of Physiology·R Eckert, A Murakami
Sep 1, 1970·Archives of Internal Medicine·T Dalhamn
Dec 1, 1965·The Annals of Otology, Rhinology, and Laryngology·C H Hakansson, N G Toremalm
Apr 1, 1956·British Journal of Industrial Medicine·T DALHAMN, J RHODIN
Jul 15, 1960·Acta Physiologica Scandinavica·T DALHAMN
Nov 10, 1962·Nature·T DALHAMN, R RYLANDER
Mar 1, 1952·British Journal of Pharmacology and Chemotherapy·P KORDIKJ H BURN

❮ Previous
Next ❯

Citations

Nov 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J M ZahmE Puchelle
Aug 1, 1989·Journal of Pharmacological Methods·P C Braga
Jun 6, 2000·Advanced Drug Delivery Reviews·F W MerkusE Marttin
Jun 1, 1993·Thorax·D VealeP A Corris
Nov 1, 1993·Acta Oto-laryngologica·L GilainE Puchelle
Sep 30, 1991·The American Journal of Medicine·L AllegraC Rampoldi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.