PMID: 6968909Aug 1, 1980Paper

Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood

Proceedings of the National Academy of Sciences of the United States of America
R A HoffmanG Goldstein

Abstract

A simple and rapid method for the determination of human T lymphocyte subclasses in buffy coat preparations or whole blood is described. This technique uses flow cytometry to distinguish lymphocytes from other leukocytes on the basis of their light-scattering properties. Lymnphocyte subclasses were enumerated by cellular immunofluorescence; the immunofluorescent signals were produced by monoclonal antibodies to surface differentiation antigens on T cells. Conventional techniques of enumerating T lymphocyte subclasses entail time-consuming (up to 2 days) density gradient and E rosette enrichement, and require at least 20 ml of blood. The method described here uses as little as 50 microliters of whole blood for each antibody tested and produces results within in 2 hr.

References

Dec 1, 1979·The Journal of Experimental Medicine·E L ReinherzS F Schlossman
Nov 8, 1979·The New England Journal of Medicine·E L ReinherzS F Schlossman
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E L ReinherzS F Schlossman
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E L ReinherzS F Schlossman

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Citations

Jan 1, 1988·Journal of Neuro-oncology·M UegakiM M Yokoyama
Mar 1, 1984·Journal of Clinical Immunology·D G PayanR H Rubin
Oct 1, 1982·Journal of Clinical Immunology·H G Herrod, W R Valenski
Apr 1, 1983·Journal of Clinical Immunology·I G Barr, B H Toh
Jan 1, 1982·Journal of Clinical Immunology·H A SampsonP J Baker
Sep 1, 1985·Journal of Clinical Immunology·F CiferriM M Glovsky
Mar 1, 1983·The Histochemical Journal·F T Bosman
Sep 1, 1994·Journal of Clinical Immunology·M C AldhousK S Froebel
Dec 28, 1999·Journal of Urban Health : Bulletin of the New York Academy of Medicine·B GreenbergD Vlahov
Dec 7, 2007·Pflügers Archiv : European journal of physiology·Raphael C ValenteMárcia A M Capella
Oct 13, 2006·Journal of Molecular Histology·Yoshihiro KomoharaAkira Yamada
Apr 8, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·P J LisiJ W Teipel
Mar 1, 1992·Developmental Biology·C TrevinoK Muneoka
Feb 25, 1983·Journal of Immunological Methods·I H Frazer, I R Mackay
Aug 12, 1983·Journal of Immunological Methods·B Freundlich, N Avdalovic
Sep 16, 1983·Journal of Immunological Methods·C F ShieldG Goldstein
Feb 24, 1984·Journal of Immunological Methods·J G GarnerR T Schooley
Mar 16, 1984·Journal of Immunological Methods·V Bongers, J Bertrams
Jan 22, 1986·Journal of Immunological Methods·J D LifsonE G Engleman
Mar 10, 1989·Journal of Immunological Methods·K TakaseJ Yata
Mar 21, 1991·Journal of Immunological Methods·J De VisM De Waele
Oct 2, 1992·Journal of Immunological Methods·J P AboulkerP Debre

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