Simple and selective high-performance liquid chromatographic method for estimating plasma quinidine levels

Journal of Chromatography
P A Reece, M Peikert

Abstract

A reversed-phase, high-performance liquid chromatographic method employing fluorescence detection is described for the rapid quantification of plasma levels of quinidine, dihydroquinidine and 3-hydroxyquinidine. It involves protein precipitation with acetonitrile followed by direct injection of the supernatant into the chromatograph. For the preparation of plasma standards, pure 3-hydroxyquinidine was isolated from human urine by a simplified thin-layer chromatographic procedure. The mobile phase for the chromatography was a mixture of 1.5 mM aqueous phosphoric acid and acetonitrile (90:10) at a flow-rate of 2 ml/min. The intra-assay coefficient of variation for the assay of quinidine and 3-hydroxyquinidine over the concentration range 2.5-20 mumole/l was less than 1% for both. Interassay coefficients of variation for quinidine (10 mumole/1) and 3-hydroxyquinidine (5 mumole/1) were 3.5% and 4.0% with detection limits of 50 and 25 mumole/l respectively. The method correlated well (r2 = 0.96) with an independently developed gas--liquid chromatographic--nitrogen detection assay for quinidine which also possessed a high degree of precision. (Intra-assay coefficient of variation 3.6% at 20 mumole/l). As expected, comparison of the h...Continue Reading

References

Jan 1, 1979·Journal of Chromatography·T W GuentertS Riegelman
Mar 28, 1974·The New England Journal of Medicine·K M KesslerM M Reidenberg
Aug 1, 1969·Biochemical Pharmacology·K H PalmerM E Wall
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Citations

Jan 1, 1988·Transactions of the Royal Society of Tropical Medicine and Hygiene·A EslavaD E Bidwell
May 10, 1985·Journal of Chromatography·M AhnoffJ Vessman
Jan 1, 1984·Journal of Pharmaceutical and Biomedical Analysis·I M Johansson, B Schubert
Oct 10, 1980·Journal of Chromatography·T W GuentertS Riegelman
Sep 12, 2015·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Li LiuJashvant D Unadkat
Jan 21, 1984·The Medical Journal of Australia·G J SchapelC J Doecke

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