PMID: 8600758Mar 1, 1996Paper

Simple and sensitive enzyme-linked immunosorbent assay for ivermectin

The American Journal of Tropical Medicine and Hygiene
Y MitsuiY Aoki

Abstract

A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) for the determination of the concentration of ivermectin (IVM) in biological fluids was developed. A conjugate of IVM on bovine serum albumin and poly-L-lysine was used to produce antibodies in rabbits and served as a solid-phase marker for titration of antibodies, respectively. The competitive ELISA was conducted by simultaneously incubating IVM and IVM-biotin conjugate with anti-IVM antiserum over goat anti-rabbit IgG (Fc) and then determining the amount of bound IVM-biotin with avidin-peroxidase conjugate as a tracer. The coefficient of variation for the assay was less than 10% in the range of 0.3-10 ng/ml. The limit of detection was 0.1 ng/ml. The cross-reactivities of anti-IVM antiserum with some anthelmintic drugs were negligible. Using this ELISA, serum levels of IVM were easily determined in Mongolian jirds (Meriones unguiculatus) up to 72 hr following a single oral dose of 500 microgram/kg of body weight.

Citations

Jun 19, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chunmei WangJianzhong Shen
Apr 18, 2002·Biosensors & Bioelectronics·Jeanne V SamsonovaChristopher T Elliott
Sep 5, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Martin DanaherMichael O'Keeffe
Aug 17, 2006·Journal of Agricultural and Food Chemistry·Weimin ShiJianzhong Shen

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