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Simple and sensitive procedure for screening yeast mutants that lyse at nonpermissive temperatures

Journal of Bacteriology

Dec 1, 1975

Enrico Cabib, Angel Durán

PMID: 372

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Abstract

After mutagenesis, surviving yeast cells are grown on plates at 25 C and later exposed to 37 C. The plates are then overlaid with a soft agar containing p-nitrophenylphosphate at pH 9.7. Lysed cells liberate alkaline phosphatase which gives rise to a yellow color on and around colonies.

Mentioned in this Paper

Hydrogen-Ion Concentration
Organopyrophosphorus Compounds
Ethyl Methanesulfonate
Alkaline Phosphatase
Saccharomyces cerevisiae
Agar
Nitrophenols
Clastogens
373
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  • Simple and sensitive procedure for screening yeast mutants that lyse at nonpermissive temperatures

    Journal of Bacteriology

    Dec 1, 1975

    Enrico Cabib, Angel Durán

    PMID: 372

    DOI:

    Abstract

    After mutagenesis, surviving yeast cells are grown on plates at 25 C and later exposed to 37 C. The plates are then overlaid with a soft agar containing p-nitrophenylphosphate at pH 9.7. Lysed cells liberate alkaline phosphatase which gives rise to a yellow color on and around colonies.

    Mentioned in this Paper

    Hydrogen-Ion Concentration
    Organopyrophosphorus Compounds
    Ethyl Methanesulfonate
    Alkaline Phosphatase
    Saccharomyces cerevisiae
    373

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