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Simple and sensitive procedure for screening yeast mutants that lyse at nonpermissive temperatures

Journal of Bacteriology

Dec 1, 1975

E Cabib, A Duran

PMID: 372

Abstract

After mutagenesis, surviving yeast cells are grown on plates at 25 C and later exposed to 37 C. The plates are then overlaid with a soft agar containing p-nitrophenylphosphate at pH 9.7. Lysed cells liberate alkaline phosphatase which gives rise to a yellow color on and around colonies.

Mentioned in this Paper

Agar
Alkaline Phosphatase
Ethyl Methanesulfonate
Organopyrophosphorus Compounds
Saccharomyces cerevisiae
Hydrogen-Ion Concentration
Nitrophenols
Clastogens
383
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Simple and sensitive procedure for screening yeast mutants that lyse at nonpermissive temperatures

Journal of Bacteriology

Dec 1, 1975

E Cabib, A Duran

PMID: 372

DOI:

Abstract

After mutagenesis, surviving yeast cells are grown on plates at 25 C and later exposed to 37 C. The plates are then overlaid with a soft agar containing p-nitrophenylphosphate at pH 9.7. Lysed cells liberate alkaline phosphatase which gives rise to a yellow color on and around colonies.

Mentioned in this Paper

Agar
Alkaline Phosphatase
Ethyl Methanesulfonate
Organopyrophosphorus Compounds
Saccharomyces cerevisiae
Hydrogen-Ion Concentration
Nitrophenols
Clastogens
383

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