PMID: 7658018Aug 18, 1995Paper

Simple assay for evaluation of Epstein-Barr virus specific cytotoxic T lymphocytes

Journal of Immunological Methods
M Okano, D T Purtilo

Abstract

Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes (CTL) are considered to be one of major defenses against the activation of EBV infection. We have developed a simple assay for evaluation of EBV-CTL by means of culturing peripheral blood mononuclear cells at the concentration of 2 x 10(6) cells/ml infected by B95-8 EBV, in the presence or absence of cyclosporin A (CSA). No lymphoblastoid cell line was established from ten EBV-seropositive healthy individuals in the absence of CSA. In contrast, cell lines were established from ten EBV-seronegative healthy individuals in the absence of CSA, indicating lack of EBV-CTL in these individuals. Methods described herein are simple and correlate well with EBV-CTL activity when compared to conventional methods, such as outgrowth regression or chromium-51 release assays.

Citations

Dec 6, 2003·The Journal of Experimental Medicine·Kara BickhamChristian Münz
Mar 27, 2001·Journal of Virology·S NikiforowG Miller
Oct 27, 2004·Journal of Translational Medicine·Nigel J WaterhouseChris Jp Clarke
Sep 24, 1999·Current Medical Research and Opinion·M Okano
Mar 1, 1997·Pediatric Hematology and Oncology·M Okano

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