Simple assay for screening phytoestrogenic compounds using the oestrogen receptor immobilised magnetite nanoparticles

IET Nanobiotechnology
Pimchanok Busayapongchai, Sineenat Siri

Abstract

With increasing interests of phytoestrogens for their potential applications, a rapid and simple tool for screening these phytochemicals is still required. In this study, a simple assay to detect phytoestrogens was developed based on the competition binding between the tested samples and the fluorescently labelled oestrogen (E2) to the human ligand binding domain of oestrogen receptor (LBD-ER) that was immobilised on the magnetite nanoparticles (MNPs). The 40-kDa LBD-ER peptide was produced in an Escherichia coli system. The synthesised 68.7-nm MNPs were silanised and subsequently covalently linked to the C-terminus of LBD-ER peptide. The LBD-ER immobilised MNPs demonstrated the specific binding for the standard E2 with the equilibrium dissociation constant of 9.56 nM and the binding capacity of 0.08 pmol/1 mg of the MNPs. The LBD-ER immobilised MNPs could evaluate oestrogenic activity of the extracts of Asparagus racemosus and Curcuma comosa, the reported phytoestrogenic plants, but not progesterone (P4) and Raphanus sativus extract, the negative controls. The results of this work clearly demonstrated a potential assay for detecting phytoestrogens of crude plant extracts, which is simple and easily adapted to a high throughput...Continue Reading

References

Sep 1, 1992·Journal of Reproduction and Fertility·A I LouwR J van Aarde
Apr 4, 2000·Toxicological Sciences : an Official Journal of the Society of Toxicology·R M BlairD M Sheehan
Aug 31, 2000·Environmental Health Perspectives·H FangD M Sheehan
Feb 13, 2001·The Journal of Steroid Biochemistry and Molecular Biology·J MatthewsT Zacharewski
May 29, 2003·The Journal of Nutrition·Mindy S Kurzer
Apr 16, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Stefan O MuellerKenneth S Korach
May 15, 2004·Molecular and Cellular Biology·Joseph J PinzonePatricia E Berg
Aug 21, 2004·Nuclear Receptor·Yasuto TaguchiDonald L Bodenner
Aug 31, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Susan C LawsJ Charles Eldridge
Jan 16, 2009·Journal of Agricultural and Food Chemistry·Wipawee WinuthayanonPawinee Piyachaturawat
Mar 30, 2010·Frontiers in Neuroendocrinology·Heather B Patisaul, Wendy Jefferson
Apr 3, 2012·BMC Complementary and Alternative Medicine·Mujalin PrasannarongApichart Suksamrarn
Oct 3, 2013·International Journal of Molecular Sciences·Raúl G LópezDarío Bueno
Mar 25, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Yuan-Ting ZhuXun Liao
Jul 30, 2014·Journal of Leukocyte Biology·Andrea E SchneiderJanos Matko
Oct 1, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qing Chang, Heqing Tang
Apr 27, 2017·Scientific Reports·Valentina CappelloMauro Gemmi

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