Simple extraction method using syringe filter for detection of ethephon in tomatoes by negative-ion mode liquid chromatography with tandem mass spectrometry

Biomedical Chromatography : BMC
Soon-Kil ChoJae-Han Shim

Abstract

In this study, a simple, rapid, and sensitive method was developed for the extraction of ethephon from homogenized tomatoes that does not require a cleanup procedure. In a syringe filter, three distinct layers - aqueous, acetonitrile, and n-hexane - are clearly separated after storage at -80 °C for 5-10 min. A Dionex IonPac column was used to separate the analyte before detection using negative-ion mode liquid chromatography with tandem mass spectrometry (LC/MS/MS). The matrix effect of the tested analyte was negligibly small and the matched calibration showed a good linearity over a concentration range of 0.01-1.0 mg/kg with a correlation coefficient (R(2) ) of 0.9998. The recovery at three fortification levels (0.1, 0.5 and 1.0 mg/kg) was between 82.9 and 108.6% with relative standard deviations (RSDs) <5.0%. The limit of quantification (0.03 mg/kg) was lower than the maximum residue limit (3 mg/kg) set by the Ministry of Food and Drug Safety, Republic of Korea. From a field trial, the method developed herein was applied to calculate the decline pattern and predict the pre-harvest residue limits of ethephon in tomatoes. In conclusion, the proposed sample preparation is feasible for the detection of hydrophilic analytes in tom...Continue Reading

References

Aug 25, 2004·Environmental Research·Peggy ReynoldsUNKNOWN California Teachers Study
Mar 17, 2010·Analytical and Bioanalytical Chemistry·Martin KraussJuliane Hollender
Oct 19, 2011·Rapid Communications in Mass Spectrometry : RCM·Jiaying XueFengmao Liu
Jun 9, 2012·Critical Care : the Official Journal of the Critical Care Forum·Duncan Wyncoll, Luigi Camporota

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