Simple Fabrication of Multicomponent Heterogeneous Fibers for Cell Co-Culture via Microfluidic Spinning

Macromolecular Bioscience
Kun YaoXinghua Gao

Abstract

Microfluidic spinning, as a combination of wet spinning and microfluidic technology, has been used to develop microfibers with special structures to facilitate cell 3D culture/co-culture and microtissue formation in vitro. In this study, a simple microchip-based microfluidic spinning strategy is presented for the fabrication of multicomponent heterogeneous calcium alginate microfibers. The use of two kinds of microchip enables the one-step preparation of multicomponent heterogeneous microfibers with various arrangement patterns, including the preparation of one-, two-, and three-component microfibers by a two-layer microchip and preparation of four component microfibers with different arrangement by a membrane-sandwiched three-layer microchip. The obtained microfibers could be used to encapsulate various kinds of cells, such as the human non-small cell lung cancer cell NCI-H1650, the human fetal lung fibroblast HFL1, the normal pulmonary bronchial epithelial cell 16HBE, and human umbilical vein endothelial cells. By adding chitosan to the medium to keep the fibers stable, 3D long-term in vitro cell co-culture has been carried out up to 21 days. This method is very simple and easy to operate, continuously produces spatially well...Continue Reading

References

Aug 15, 2009·Lab on a Chip·Jae-Hoon JungChang-Soo Lee
Jan 27, 2010·Biotechnology Advances·Nandana Bhardwaj, Subhas C Kundu
Feb 5, 2010·Nature·Yongmei ZhengLei Jiang
Mar 26, 2011·Advanced Materials·Willie WuJennifer A Lewis
May 10, 2012·Lab on a Chip·Janine K NunesHoward A Stone
Jun 29, 2012·Advanced Materials·Edward KangSang-Hoon Lee
Apr 2, 2013·Nature Materials·Hiroaki OnoeShoji Takeuchi
Jun 12, 2013·Tissue Engineering. Part C, Methods·Joshua HammerFan Yang
Jan 24, 2014·Advanced Materials·Yue YuJianhua Qin
Jun 18, 2014·Advanced Materials·Yao ChengZhongze Gu
Mar 21, 2015·Acta Biomaterialia·Roman A Perez, Hae-Won Kim
May 18, 2016·Advanced Materials·Yue YuJianhua Qin
Nov 18, 2017·Macromolecular Bioscience·Marilyn C McNamaraNicole N Hashemi
Feb 21, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Hui LiuJianhua Qin
Apr 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gen WangBin Li
Jul 10, 2019·Advanced Materials·Haitao LiuJianhua Qin
Sep 11, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Hui LiuJianhua Qin
Jun 14, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ming-Yue JiangLiang-Yin Chu
Sep 10, 2018·ACS Biomaterials Science & Engineering·Yujuan ZhuJianhua Qin

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