Simple, fast preparation of gallium-68-labelled human serum albumin microspheres

European Journal of Nuclear Medicine
J P YvertD Comar

Abstract

Following a study of the main factors involved in the 68-Ga labelling of human serum albumin microspheres (H.S.A.M.), especially methods of production and preparation of active solution and conditions of radioelement fixation on the protein support, the practical details of a fast technique (60 min) based on the process described by Hnatowich are presented. This method gives high labelling yields (93 +/- 3%), and after washing of the microspheres leads to a radiopharmaceutical product almost without free 68Ga (less than 2%). The spheres ready for use carry a total radioactivity corresponding to about 35%, including decay, of the activity originally recovered in the generator eluate and to more than 98% of that, found in the final suspension. The labelled product is sterile, non-pyrogenic and non-toxic. When it is injected in animals by left ventrical catheterization the uptake rates in the heart, lungs, spleen, left kidney and right kidney are similar to those observed with reference 85Sr-labelled carbonized microspheres. This radiopharmaceutical, easy to prepare and having excellent biological and nuclear properties, seems ideally suited for the scanning of organs by position emission tomoscintigraphy.

References

Oct 1, 1989·Journal of Microencapsulation·P K Gupta, C T Hung
Nov 9, 2010·Nuclear Medicine and Biology·G WunderlichHans-Jürgen Pietzsch
Jul 22, 2008·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Carla J Mathias, M A Green

Citations

Jan 1, 1977·The International Journal of Applied Radiation and Isotopes·S Kulprathipanja, D J Hnatowich
Jan 1, 1977·The International Journal of Applied Radiation and Isotopes·D J Hnatowich
Apr 1, 1975·The International Journal of Applied Radiation and Isotopes·R T GouletA A Noujaim
Dec 1, 1975·Radiology·A D WaxmanJ K Siemsen
Jan 1, 1971·The International Journal of Applied Radiation and Isotopes·J E Carlton, R L Hayes
Mar 1, 1970·The International Journal of Applied Radiation and Isotopes·I ZolleH N Wagner
Mar 1, 1971·The International Journal of Applied Radiation and Isotopes·R C BarrallR A Finston
Jul 1, 1960·The International Journal of Applied Radiation and Isotopes·G I GLEASON

Related Concepts

Strontium Radioisotopes
Lung
Spleen
Isotope Labeling, Stable
ALB
Uptake
Albumin Human, USP
Gallium Radioisotopes
Sample Fixation
Human Serum Albumin [EPC]

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Related Papers

International Journal of Radiation Applications and Instrumentation. Part A, Applied Radiation and Isotopes
B MaziereD Comar
European Journal of Nuclear Medicine
R L HayesY Kuniyasu
The International Journal of Applied Radiation and Isotopes
L J Anghileri
© 2020 Meta ULC. All rights reserved