Simple high-performance liquid chromatographic method for the determination of all seven vitamin B6-related compounds

Journal of Chromatography. a
C J Argoudelis

Abstract

A simple, sensitive, isocratic high-performance liquid chromatographic method has been developed for the separation of all seven vitamin B6-related compounds. The separation is accomplished using an ODS column and a mobile phase of 0.15 M sodium dihydrogenphosphate, adjusted to pH 2.5 with perchloric acid. The concentration of the compounds is determined with a fluorescence detector (excitation, 290 nm; emission, 389 nm). Isopyridoxal is used as an internal standard. The fluorescence intensity of pyridoxal-5'-phosphate is enhanced by post-column derivatization with sodium bisulfite. All seven compounds are separated in less than 20 min at a flow-rate of 1 ml/min. Applications of this method to yeast cell-free culture media, baker's yeast extract, egg and milk are presented.

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Citations

Jul 22, 2010·The Journal of General and Applied Microbiology·Chitchanok AnutrakunchaiYanee Trongpanich
Sep 8, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Marcin L MarszałłPiotr Szefer
Jan 1, 2005·Annali Di Chimica·Mohammad Reza HadjmohammadiJafar Hoseinalizade Khorasani
May 8, 2016·Talanta·Oliver Wahl, Ulrike Holzgrabe
Jun 19, 2004·The International Journal of Biochemistry & Cell Biology·M M Mahfouz, F A Kummerow
Feb 23, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·C J Argoudelis
Jun 29, 1999·Analytical Chemistry·D J Anderson

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