Simple in Vivo Gene Editing via Direct Self-Assembly of Cas9 Ribonucleoprotein Complexes for Cancer Treatment

ACS Nano
Seung Min KimMihue Jang

Abstract

Cas9 ribonucleoprotein (RNP)-mediated delivery has emerged as an ideal approach for in vivo applications. However, the delivery of Cas9 RNPs requires electroporation or lipid- or cationic-reagent-mediated transfection. Here, we developed a carrier-free Cas9 RNP delivery system for robust gene editing in vivo. For simultaneous delivery of Cas9 and a guide RNA into target cells without the aid of any transfection reagents, we established a multifunctional Cas9 fusion protein (Cas9-LMWP) that forms a ternary complex with synthetic crRNA:tracrRNA hybrids in a simple procedure. Cas9-LMWP carrying both a nuclear localization sequence and a low-molecular-weight protamine (LMWP) enables the direct self-assembly of a Cas9:crRNA:tracrRNA ternary complex (a ternary Cas9 RNP) and allows for the delivery of the ternary Cas9 RNPs into the recipient cells, owing to its intrinsic cellular and nuclear translocation ability with low immunogenicity. To demonstrate the potential of this system, we showed extensive synergistic anti-KRAS therapy (CI value: 0.34) via in vitro and in vivo editing of the KRAS gene by the direct delivery of multifunctional Cas9 RNPs in lung cancer. Thus, our carrier-free Cas9 RNP delivery system could be an innovative p...Continue Reading

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Citations

Nov 24, 2019·Advanced Drug Delivery Reviews·Cong-Fei XuJun Wang
Jul 28, 2020·Advanced Drug Delivery Reviews·Fangli MoWeihong Tan
Sep 16, 2020·Advanced Drug Delivery Reviews·Jie LiNiren Murthy
May 4, 2020·Advanced Drug Delivery Reviews·Xiangrong SongYuquan Wei
May 18, 2020·Advanced Drug Delivery Reviews·Tao Wan, Yuan Ping
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Feb 2, 2022·Biomaterials Science·Yi LinUlrich Lächelt

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