Simple limbal epithelial transplantation (SLET): a novel surgical technique for the treatment of unilateral limbal stem cell deficiency

The British Journal of Ophthalmology
Virender S SangwanDorairajan Balasubramanian

Abstract

This study describes a novel surgical technique of limbal transplantation, which combines the benefits of existing techniques while avoiding their difficulties. Six patients with unilateral and total limbal stem cell deficiency following ocular surface burns underwent a single-stage procedure. A 2 × 2 mm strip of donor limbal tissue was obtained from the healthy eye and divided into eight to ten small pieces. After surgical preparation of the recipient ocular surface, these tiny limbal transplants were distributed evenly over an amniotic membrane placed on the cornea. After surgery, a completely epithelialised, avascular and stable corneal surface was seen in all recipient eyes by 6 weeks, and this was maintained at a mean ± SD follow-up of 9.2 ± 1.9 months. Visual acuity improved from worse than 20/200 in all recipient eyes before surgery to 20/60 or better in four (66.6%) eyes, while none of the donor eyes developed any complications. This technique requires less donor tissue than previously used for conventional autografting and does not need a specialist laboratory for cell expansion. Although long-term results are awaited, this simple limbal epithelial transplantation promises to be an easy and effective technique for trea...Continue Reading

Associated Clinical Trials

Citations

Mar 28, 2013·Journal of Ophthalmology·Shivalingappa K Swamynathan
Apr 13, 2013·Middle East African Journal of Ophthalmology·Kunjal SejpalSophie X Deng
Mar 13, 2014·Acta Biomaterialia·Enas HassanSheila MacNeil
Jul 19, 2015·Progress in Retinal and Eye Research·Takahiro NakamuraShigeru Kinoshita
May 30, 2013·Cutaneous and Ocular Toxicology·Ayse BurcuFirdevs Ornek
Feb 7, 2015·Current Eye Research·Benny BjörkblomSten Raeder
Apr 2, 2013·Progress in Retinal and Eye Research·John V ForresterMatthew E Wikstrom
Mar 5, 2016·Ocular Immunology and Inflammation·Ashik Mohamed, Virender S Sangwan
Jan 21, 2016·Stem Cells International·Michel HaagdorensNadia Zakaria
Mar 10, 2016·Seminars in Ophthalmology·Haumith Khan-Farooqi, James Chodosh
Aug 25, 2015·American Journal of Ophthalmology·Carl ShenEdward J Holland
Oct 10, 2015·BioMed Research International·Beatriz E RamírezMargarita Calonge
Jun 18, 2015·BMJ Case Reports·Jayesh VaziraniVirender Sangwan
Apr 20, 2016·Regenerative Medicine·Graziella PellegriniPaolo Rama
Oct 4, 2014·Saudi Journal of Ophthalmology : Official Journal of the Saudi Ophthalmological Society·Hong He, Samuel C Yiu
Jun 18, 2016·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Peng ChenLixin Xie
Jun 3, 2016·Stem Cell Research & Therapy·Jeremy John MathanTrevor Sherwin
May 7, 2016·The Ocular Surface·Jayesh VaziraniVirender S Sangwan
Aug 9, 2016·Regenerative Medicine·Matthias FuestJodhbir S Mehta
Aug 3, 2016·The Ocular Surface·Jennifer RossenAli Djalilian
Nov 12, 2016·American Journal of Ophthalmology·Swathi KalikiVirender S Sangwan
Jan 29, 2016·The British Journal of Ophthalmology·Jayesh VaziraniJames Chodosh
Sep 22, 2016·The Ocular Surface·Alireza Baradaran-RafiiAli R Djalilian
Sep 11, 2014·Stem Cells Translational Medicine·Charanya RamachandranDorairajan Balasubramanian
Apr 14, 2017·Current Opinion in Ophthalmology·Virender S Sangwan, John A H Sharp
Apr 21, 2017·Current Opinion in Ophthalmology·Alfonso L Sabater, Victor L Perez
Apr 12, 2017·Current Opinion in Ophthalmology·Sheraz M Daya
Mar 9, 2017·Biomedical Optics Express·René M WerkmeisterLeopold Schmetterer
Jul 13, 2017·Ocular Immunology and Inflammation·Alfonso Vasquez-Perez, Mayank A Nanavaty
Nov 27, 2016·The British Journal of Ophthalmology·Harminder S DuaDalia G Said
Jul 18, 2017·Clinical & Experimental Ophthalmology·Minas T Coroneo
Feb 24, 2018·Expert Opinion on Biological Therapy·Yuzuru SasamotoNatasha Y Frank
Mar 25, 2016·World Journal of Transplantation·Tian YuLucia Kuffová
Dec 7, 2017·Current Opinion in Ophthalmology·Virender S SangwanShilpa Das
Apr 14, 2017·The British Journal of Ophthalmology·Geetha IyerAnil Tarigopula

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