Simple liquid chromatography-electrospray ionization ion trap mass spectrometry method for the quantification of galacto-oxylipin arabidopsides in plant samples.

Scientific Reports
Manon GenvaMarie-Laure Fauconnier

Abstract

A simple and sensitive method to quantify five different arabidopsides by HPLC-ion trap mass spectrometry in complex plant samples was developed and validated. Arabidopsides are oxidized galactolipids first described in Arabidopsis thaliana but also produced by other plant species under stress conditions. External calibration was performed using arabidopsides purified from freeze-thawed Arabidopsis leaves. Lipids were extracted and pre-purified on an SPE silica column before HPLC-MS analysis. Arabidopsides were separated on a C18 column using a gradient of mQ water and acetonitrile:mQ water (85:15) supplemented with formic acid (0.2%) and ammonium formate (12 mM). The method was validated according to European commission decision 2002/657/CE. LOD, LOQ, linearity, intra-day and inter-day precision and accuracy, selectivity, matrix effects and recoveries were determined for the five metabolites. The established method is highly selective in a complex plant matrix. LOD and LOQ were, respectively, in the range 0.098-0.78 and 0.64-1.56 µM, allowing the arabidopside quantification from 25.6-62.4 nmol/g fresh weight. Calibration curve correlation coefficients were higher than 0.997. Matrix effects ranged from -2.09% to 6.10% and recov...Continue Reading

References

Mar 14, 2003·FEBS Letters·Marie-Laure FauconnierRuth Welti
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Apr 23, 2005·Journal of Natural Products·Yosuke HisamatsuHideyuki Shigemori
Jan 31, 2006·The Plant Journal : for Cell and Molecular Biology·Martin J MuellerEdward E Farmer
Apr 4, 2007·Planta·Christine Böttcher, Elmar W Weiler
Oct 24, 2007·Plant Physiology·Olga KourtchenkoMats Ellerström
Jun 10, 2008·Plant Physiology and Biochemistry : PPB·Marie-Laure FauconnierPatrick du Jardin
Jan 1, 2011·Frontiers in Plant Science·Amina IbrahimIvo Feussner
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Aug 29, 2018·International Journal of Molecular Sciences·Claus Wasternack, Miroslav Strnad
Nov 2, 2019·Trends in Plant Science·Estelle DeboeverMarie-Laure Fauconnier

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Citations

Dec 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gregorio PeronStefano Dall'Acqua
Mar 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu SongRuth Welti

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Methods Mentioned

BETA
infrared spectroscopy

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