Simple low cost differentiation of Candida auris from Candida haemulonii complex using CHROMagar Candida medium supplemented with Pal's medium

Revista iberoamericana de micología
Anil KumarShamsul Karim

Abstract

Candida auris is unique due to its multidrug resistance and misidentification as Candida haemulonii by commercial systems. Its correct identification is important to avoid inappropriate treatments. To develop a cheap method for differentiating C. auris from isolates identified as C. haemulonii by VITEK2. Fifteen C. auris isolates, six isolates each of C. haemulonii and Candida duobushaemulonii, and one isolate of Candida haemulonii var. vulnera were tested using CHROMagar Candida medium supplemented with Pal's agar for better differentiation. On CHROMagar Candida medium supplemented with Pal's agar all C. auris strains showed confluent growth of white to cream colored smooth colonies at 37°C and 42°C after 24 and 48h incubation and did not produce pseudohyphae. The isolates of the C. haemulonii complex, on the contrary, showed poor growth of smooth, light-pink colonies at 24h while at 48h the growth was semiconfluent with the production of pseudohyphae. C. haemulonii complex failed to grow at 42°C. We report a rapid and cheap method using CHROMagar Candida medium supplemented with Pal's agar for differentiating C. auris from isolates identified as C. haemulonii by VITEK2.

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Citations

Nov 17, 2017·Clinical Microbiology Reviews·Anna Jeffery-SmithColin S Brown
Nov 24, 2017·Journal of Clinical Microbiology·Emily S Spivak, Kimberly E Hanson
Sep 7, 2017·Expert Opinion on Drug Discovery·Tsun Sheng N KuSamuel A Lee
Jun 7, 2019·Archives of Pathology & Laboratory Medicine·D Jane HataUNKNOWN College of American Pathologists Microbiology Committee
Jul 17, 2020·Journal of Paediatrics and Child Health·Jayasree ChandramatiSasidharan Ponthenkandath
Dec 22, 2019·Journal of Fungi·Gustavo FontechaSharleen Braham
Sep 5, 2019·Frontiers in Microbiology·Milena Kordalewska, David S Perlin
Oct 23, 2020·PLoS Pathogens·Han DuGuanghua Huang
Nov 20, 2020·Journal of Clinical Microbiology·Sourav DasAnup Ghosh
Dec 23, 2020·Journal of Fungi·Irene Jurado-MartínElena Eraso
May 1, 2021·International Journal of Molecular Sciences·Lucia ČernákováCélia F Rodrigues

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