Simple method for determining biovar and serovar types of Ureaplasma urealyticum clinical isolates using PCR-single-strand conformation polymorphism analysis

Journal of Clinical Microbiology
D PitcherJ A Robertson

Abstract

Ureaplasma urealyticum has been associated with urethritis in men, obstetric problems in women, and respiratory distress syndrome in preterm infants. U. urealyticum can be divided into two biovars comprising 14 serovars. Partial sequences of genes encoding the multiple-banded antigens of the cell surface are known. Using a commercially available precast DNA mutation detection gel system, we have developed a simple and reproducible PCR-single-strand conformation polymorphism analysis method for differentiating the biovars of this species that reveals five patterns among the 14 serovars and enables clinical isolates to be typed directly from broth cultures.

References

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Citations

Mar 21, 2009·Transplantation·Alicja M EkielGayane Martirosian
Oct 15, 2005·Clinical Microbiology Reviews·Ken B WaitesRobert L Schelonka
Jun 18, 2010·Journal of Clinical Microbiology·Li XiaoKen B Waites
Feb 24, 2007·Seminars in Perinatology·Robert L Schelonka, Ken B Waites
Dec 2, 2009·Journal of Korean Medical Science·Alicja M EkielGayane Martirosian
Jun 28, 2008·Scandinavian Journal of Infectious Diseases·Alicja M EkielGayane Martirosian
Nov 20, 2002·World Journal of Gastroenterology : WJG·Ji-Cheng LiMin-Ju Li

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