Simple method for screening of alpha-thalassaemia 1 carriers

International Journal of Hematology
Chatchai TayapiwatanaWatchara Kasinrerk

Abstract

Alpha-thalassaemia 1 genetic disorder occurs when there is a deletion of two linked alpha-globin genes. The interaction between these abnormal genes leads to the most severe type of thalassaemia disease, haemoglobin (Hb) Bart's hydrops fetalis. The identification of alpha-thalassaemia 1 carriers and genetic counselling are essential for the prevention and control of severe thalassaemia diseases. In this study, we have developed a rapid screening method for identifying alpha-thalassaemia 1. A sandwich-type immunochromatographic (IC) strip test was developed, using the generated monoclonal anti-Hb Bart's antibody, to trace the Hb Bart's in haemolysates. When assayed by our IC strip test, all alpha-thalassaemia 1, HbH disease, HbH-Constant Spring (H-CS) disease, HbH-CS and heterozygous HbE (CSEA) Bart's disease, and homozygous alpha-thalassaemia 2 showed positive results. No false negative results were observed in these blood samples. In alpha-thalassaemia 2 heterozygotes, 83% of them showed positive reactivity. Among HbE (both homozygotes and heterozygotes), beta-thalassaemia (heterozygotes, homozygotes and beta-thalassaemia/HbE) and normal subjects, the IC strip test revealed negative reactivity of 100, 85 and 97%, respectively....Continue Reading

References

Jan 1, 1979·Hemoglobin·P WasiP Winichagoon
Nov 1, 1971·Immunochemistry·W P Faulk, G M Taylor
Apr 17, 1998·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·Chanane WanapirakA Tuggapichitti
Oct 6, 2000·Methods : a Companion to Methods in Enzymology·S H PaekY S Kim
May 10, 2002·International Journal of Hematology·Tyen-Po ChenSheng-Fung Lin
Nov 25, 2004·Hematology·Alan R CohenElliott Vichinsky
Jul 15, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Su-Hua Huang
Nov 23, 2006·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Luksana MakonkawkeyoonSanit Makonkawkeyoon
Oct 31, 2008·Talanta·Supaporn Kradtap HartwellKate Grudpan

Citations

Jul 30, 2009·Cytotechnology·Napaporn ApiratmateekulWatchara Kasinrerk
Jan 13, 2010·International Journal of Hematology·Surakit KuntarukWatchara Kasinrerk
Aug 27, 2010·International Journal of Hematology·Sawitree ChiampanichayakulWatchara Kasinrerk
Oct 4, 2011·Indian Journal of Clinical Biochemistry : IJCB·Vijay S BhatPatnam Rajagopalan Krishnaswamy
Dec 4, 2013·Critical Reviews in Clinical Laboratory Sciences·Ho-Wan Ip, Chi-Chiu So
Dec 4, 2013·Journal of Immunoassay & Immunochemistry·Supansa PataWatchara Kasinrerk
Jun 8, 2014·Journal of Medical Screening·Patcharawadee PrayalawSupan Fucharoen
Jul 10, 2018·Journal of Immunoassay & Immunochemistry·Sasiwan KerdpooThanusak Tatu
Aug 18, 2018·International Journal of Laboratory Hematology·Carolyn BunkallGeorge Chan
Aug 30, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Patcharapong PantiyaSakorn Pornprasert
Sep 24, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Sakorn Pornprasert
Apr 5, 2013·Prenatal Diagnosis·Theera TongsongSavitree Pranpanus
Feb 9, 2021·Clinical Chemistry and Laboratory Medicine : CCLM·Paweena YanpanyaSakorn Pornprasert

Related Concepts

Hemoglobin Bart's
Monoclonal Antibodies
Chromatography
Abnormal Hemoglobins
Genetic Carriers
Immunoassay Method
In Vitro Diagnostic Devices
Alpha-Thalassemia
Monoclonal Antibodies
Beta Thalassemia

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

Related Papers

The Indian Journal of Medical Research
Suthat Fucharoen, P Winichagoon
The Journal of Molecular Diagnostics : JMD
Thongperm MunkongdeeSaovaros Svasti
Annals of Hematology
Kanokwan SanchaisuriyaPattara Sanchaisuriya
© 2021 Meta ULC. All rights reserved