PMID: 9421499Feb 28, 1998Paper

Simple miniaturized gel system for DNA sequence analysis

Nucleic Acids Research
A SteinM Bina

Abstract

A simple miniaturized gel system suitable for DNA sequencing is described. Small ultrathin polyacrylamide gels are cast, eight or more at a time, using standard microscope slides. Gels, ready to use, can be stored for approximately 2 weeks. Gels are run horizontally in a standard mini-agarose gel apparatus. Typical run times are 6-8 min. A novel sample loading system permits volumes of standard sequencing reactions as small as 0.1 microl to be analyzed. Sequencing ladders were visualized using 35S-labeled DNA by autoradiography and by colorimetric detection. Band resolution compares favorably with that of large gels. The methods introduced here serve as a step toward the miniaturization of DNA sequencing and are amenable to automated sample loading and detection.

References

Aug 21, 1991·Nucleic Acids Research·R L Brumley, L M Smith
Mar 25, 1990·Nucleic Acids Research·H Swerdlow, R Gesteland
May 1, 1990·Analytical Chemistry·H DrossmanL M Smith
Mar 1, 1984·Journal of Biochemical and Biophysical Methods·W Ansorge, R Barker
Aug 1, 1981·Analytical Biochemistry·H Garoff, W Ansorge
Jan 1, 1993·Methods in Enzymology·P Richterich, G M Church
Oct 15, 1995·Analytical Chemistry·A T Woolley, R A Mathies

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Citations

Feb 24, 2001·Electrophoresis·A Guttman, Z Rónai
Sep 18, 2013·Applied Biochemistry and Biotechnology·Taichi E TakasukaArnold Stein
Sep 11, 2013·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Kushani ShahArnold Stein

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