Simple one-step procedure for the separation of apolipoproteins A1 and A2 by high-performance liquid chromatography

Journal of Chromatography. B, Biomedical Sciences and Applications
Z Xiong

Abstract

A simple assay method for apolipoproteins apo A1 and apo A2 by HPLC is introduced. The simple one-step method is based on fractionation of apo A1 and apo A2 from other serum proteins which are precipitated at 100 degrees C and removed by centrifugation. The apo A1 and apo A2 which remain in solution can be recovered and resolved by size-exclusion chromatography without ultracentrifugation and delipidation by an organic solvent. This makes sample preparation easier. The recoveries of apo A1 and apo A2 were 104.26% and 101.04%; the precision (C.V.%) of apo A1 and apo A2 was 0.88 and 1.63 respectively.

References

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Jan 1, 1986·Methods in Enzymology·J C Osborne
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